BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0826700 Os03g0826700|AK071165
         (250 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G01470.2  | chr5:190953-192462 FORWARD LENGTH=246              280   7e-76

>AT5G01470.2 | chr5:190953-192462 FORWARD LENGTH=246
          Length = 245

 Score =  280 bits (715), Expect = 7e-76,   Method: Compositional matrix adjust.
 Identities = 143/252 (56%), Positives = 183/252 (72%), Gaps = 12/252 (4%)

Query: 1   METALFSAASLFRXXXXXXXXXXXXXXXXXXSVQYEERAHKFPGMELKIREFSSHQLNAN 60
           M+ ALFS ASLF                   S  + ER H+FPG+EL+IREF  HQLNAN
Sbjct: 1   MDIALFSPASLF-----AADGDSSDGETTETSQNFVERNHQFPGIELQIREFGFHQLNAN 55

Query: 61  MLWPGTFLFADWLVKNKSILHGRRILELGSGTGALAIFLRKAFQVDITTTDYDDGEIQEN 120
           +LWPGTF FADWL++++ ++  RR LE+GSGTGALAIFL+K F +DITT+DY+D EI++N
Sbjct: 56  LLWPGTFAFADWLLQHRYLIERRRCLEIGSGTGALAIFLKKEFNLDITTSDYNDQEIEDN 115

Query: 121 IAYNCKAND-LGVLPHIRHTWGDQFPVLIPDWDIVIASDILLYVKQYPNLTRTLSFLLKE 179
           I +NC AN  +  LPHI+HTWGD+FP+  PDWD++IASDILLYVKQYPNL ++L+FLLK+
Sbjct: 116 IVHNCIANKIIPSLPHIKHTWGDEFPISEPDWDLIIASDILLYVKQYPNLIKSLTFLLKK 175

Query: 180 YKGCSQNAGSSASTAITNKSGTQVPVKFPIFLMSCRRRIGK-DQSLFFEECEKAGLEVQH 238
           YK    N  S A   + N + T++P   P+FLMS RRRIGK D+SLFF  CE+AGLEV+H
Sbjct: 176 YK--PTNVVSPAEGKL-NGADTELPR--PVFLMSWRRRIGKDDESLFFTGCEEAGLEVKH 230

Query: 239 LGALVYLIYTKQ 250
           LG  VY I  ++
Sbjct: 231 LGNRVYCIKLRE 242



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.137    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,825,289
Number of extensions: 193806
Number of successful extensions: 457
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 456
Number of HSP's successfully gapped: 2
Length of query: 250
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 154
Effective length of database: 8,474,633
Effective search space: 1305093482
Effective search space used: 1305093482
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 110 (47.0 bits)