BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0784500 Os03g0784500|AK108472
(273 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT2G34730.1 | chr2:14654611-14657443 FORWARD LENGTH=826 70 2e-12
>AT2G34730.1 | chr2:14654611-14657443 FORWARD LENGTH=826
Length = 825
Score = 69.7 bits (169), Expect = 2e-12, Method: Compositional matrix adjust.
Identities = 53/151 (35%), Positives = 88/151 (58%), Gaps = 8/151 (5%)
Query: 108 LNSSLEIASAALKEV--ENKNIDYNGIFTKNEQE---KQLECI-LISIMKLSKEFVEIEQ 161
L LE+A +LKE+ E + + TK E+E KQL + L+ +L K F +E
Sbjct: 657 LREELELARESLKEMKDEKRKTEEKLSETKAEKETLKKQLVSLDLVVPPQLIKGFDILEG 716
Query: 162 KLS--VERSASRSEDLSDHCNHMVRQAVVLTKIGLWYKQMLETRRSELQKAEAKVVILGD 219
++ +++ SR +++ + + Q + YKQ LE + +L+KAEA+V +LGD
Sbjct: 717 LIAEKTQKTNSRLKNMQSQLSDLSHQINEVKGKASTYKQRLEKKCCDLKKAEAEVDLLGD 776
Query: 220 KVNSHLNLLQKIYVTLDRYSPTLQQYPGLLD 250
+V + L+LL+KIY+ LD YSP L+ YPG+++
Sbjct: 777 EVETLLDLLEKIYIALDHYSPILKHYPGIIE 807
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.312 0.128 0.341
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,304,116
Number of extensions: 204770
Number of successful extensions: 931
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 941
Number of HSP's successfully gapped: 2
Length of query: 273
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 176
Effective length of database: 8,447,217
Effective search space: 1486710192
Effective search space used: 1486710192
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 111 (47.4 bits)