BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0747300 Os03g0747300|AK071214
         (282 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G17420.1  | chr4:9722544-9724443 REVERSE LENGTH=286            369   e-103
AT5G47420.1  | chr5:19233013-19234838 FORWARD LENGTH=283          362   e-101

>AT4G17420.1 | chr4:9722544-9724443 REVERSE LENGTH=286
          Length = 285

 Score =  369 bits (947), Expect = e-103,   Method: Compositional matrix adjust.
 Identities = 180/286 (62%), Positives = 227/286 (79%), Gaps = 5/286 (1%)

Query: 1   MAAPFFSTPFQPYVYQSQEGSVTAFQISGGDVQVLQVMVKSQEKLTVKPGTMCYMSGNIQ 60
           MAAPFFSTPFQPYVYQSQE ++T FQI GG+ QV+Q+M+K QEK+  KPG+MCYMSG+I+
Sbjct: 1   MAAPFFSTPFQPYVYQSQEDTITPFQILGGEAQVVQIMLKPQEKVIAKPGSMCYMSGSIE 60

Query: 61  TDNNYLPENDGGVWQWIFGKSISSSVFFNPGSDDGYVGISAPFPGRILPMDLANFGGELL 120
            DN Y PE + GV QWI GKS+SS V  N G +DG+VGI+AP   RILP+DLA FGG++L
Sbjct: 61  MDNTYTPEQEVGVVQWILGKSVSSIVLRNTGQNDGFVGIAAPSLARILPLDLAMFGGDIL 120

Query: 121 CQADAFLCSVNDVSVTSTVEQRPRNIEI---GAEVILKQKLRGQGMAFLVGGGSVMQKIL 177
           CQ DAFLCSV+DV V +TV QR R   I   GAE +L+Q+L GQG+AF++ GGSV+QK L
Sbjct: 121 CQPDAFLCSVHDVKVVNTVYQRHRARNIAAAGAEGVLRQRLSGQGLAFIIAGGSVVQKNL 180

Query: 178 APREVITVDAACIVAMTTTINFQLKTPNQP-RRVVFSGGNQLTASLTGPGVVFIQSLPFH 236
              EV+T+D +CI A+T +INFQ+K    P RR VF G N +TA+LTGPG+VFIQSLPFH
Sbjct: 181 EVGEVLTIDVSCIAALTPSINFQIKYNAAPVRRAVFGGDNVVTATLTGPGIVFIQSLPFH 240

Query: 237 RLSQRIASRSVAAPSLRDNPKFFIQIVMFFFLAYVMIVSSIILTDV 282
           RLSQRIA RSV +P++R+NP+  +QI +F FLAYV+IVSS+ILT++
Sbjct: 241 RLSQRIA-RSVTSPNMRENPRLLVQIGLFIFLAYVVIVSSLILTEM 285



>AT5G47420.1 | chr5:19233013-19234838 FORWARD LENGTH=283
          Length = 282

 Score =  362 bits (930), Expect = e-101,   Method: Compositional matrix adjust.
 Identities = 172/283 (60%), Positives = 225/283 (79%), Gaps = 2/283 (0%)

Query: 1   MAAPFFSTPFQPYVYQSQEGSVTAFQISGGDVQVLQVMVKSQEKLTVKPGTMCYMSGNIQ 60
           MAAPFFSTPFQPYVYQSQ+ ++T FQI GG+ QV+Q+M+KS+EK+  KP +MCYMSG+I+
Sbjct: 1   MAAPFFSTPFQPYVYQSQQDTITPFQILGGESQVVQIMLKSEEKVIAKPASMCYMSGSIE 60

Query: 61  TDNNYLPENDGGVWQWIFGKSISSSVFFNPGSDDGYVGISAPFPGRILPMDLANFGGELL 120
            +N Y PE + GV QWI GKS+SS V  N G +DG+VGI+AP+  RILP+DLA FGGE+L
Sbjct: 61  MENTYTPEQEVGVLQWILGKSVSSVVLRNTGQNDGFVGIAAPYLARILPIDLAMFGGEIL 120

Query: 121 CQADAFLCSVNDVSVTSTVEQRPRNI-EIGAEVILKQKLRGQGMAFLVGGGSVMQKILAP 179
           CQ DAFLCSV+DV V ++V+QR RNI   GAE  L+Q+L GQG+AF++ GGSV+QK+L  
Sbjct: 121 CQPDAFLCSVHDVKVVNSVDQRARNIVAAGAEGFLRQRLSGQGLAFILAGGSVVQKVLEV 180

Query: 180 REVITVDAACIVAMTTTINFQLKTPNQPRRVVFSGGNQLTASLTGPGVVFIQSLPFHRLS 239
            EV ++D +CI A+T +I+ ++K     RR +F G N + A+LTGPG+VFIQSLPFHRLS
Sbjct: 181 GEVFSIDVSCIAALTPSIDVRIKNNAPFRRALFGGDNVVMATLTGPGIVFIQSLPFHRLS 240

Query: 240 QRIASRSVAAPSLRDNPKFFIQIVMFFFLAYVMIVSSIILTDV 282
           QRIA RSV +P++R+NPK  IQI +F FLAY +I+SS+ILT+V
Sbjct: 241 QRIA-RSVTSPNMRENPKLLIQIAIFAFLAYAVILSSLILTEV 282



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.137    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,941,794
Number of extensions: 245768
Number of successful extensions: 489
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 484
Number of HSP's successfully gapped: 2
Length of query: 282
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 185
Effective length of database: 8,447,217
Effective search space: 1562735145
Effective search space used: 1562735145
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 111 (47.4 bits)