BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0726900 Os03g0726900|AK072553
         (308 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G65740.2  | chr5:26301702-26303422 REVERSE LENGTH=301          322   2e-88

>AT5G65740.2 | chr5:26301702-26303422 REVERSE LENGTH=301
          Length = 300

 Score =  322 bits (824), Expect = 2e-88,   Method: Compositional matrix adjust.
 Identities = 152/284 (53%), Positives = 208/284 (73%), Gaps = 6/284 (2%)

Query: 29  RPAAFYSAVFAQIEEVGWERLVSGKGDGGVSCLVFRILDDQGRNHLLEITLPMNYPSSPP 88
           + ++FY  V+++IEE+GWE +    GD  ++   F ILD +GR H LEI L  +YP+SPP
Sbjct: 19  KSSSFYRKVYSEIEEIGWEPIRRLGGD--LTFFSFHILDKKGRAHNLEIQLNRDYPNSPP 76

Query: 89  CLVADVPYLPELQWSKGSRLKDVVCQFQEHLKILQDYWSIMDDIDKVLWVVDPTKPTFAM 148
            + ADVPY+  L+WS  SRLKDV+ QFQ+HL  LQ++WS++D+IDK L VVD  +P  A 
Sbjct: 77  SVSADVPYMFTLEWSTSSRLKDVMHQFQKHLDYLQEFWSVLDNIDKSLCVVDVKQPARAS 136

Query: 149 SHRRIALGDDCYLLLHVNVRKPRSLPEVRFLG---TDGKLDRLITNWRKYCKKWSADKKF 205
           + RRI  G+DC +++H++ + P+SLPE RF+G   +   ++ L   WR+ CK+WS ++ F
Sbjct: 137 AIRRIDAGNDCIIIVHIDFKDPKSLPESRFIGPVPSATHMNNLHMLWRRNCKRWSNERSF 196

Query: 206 HENLSTVLDFALPPPPSVNIEDDEQ-VDCGICYAKHLPIDDELGTHSGGTTDYTCENPSC 264
            ENL  +L   LP P  + +EDD+Q V+CGICYA+ LP D+ELG  SG  TDYTCEN SC
Sbjct: 197 PENLECILGTELPKPLGLQVEDDQQQVECGICYAQFLPTDEELGARSGTRTDYTCENISC 256

Query: 265 SRAFHSVCLRDWLRAITTTRQSFDVLFGNCPYCSDPVAVKITDR 308
           +++FHS+CL DWLR+ITTTRQSFDVLFGNCPYCSDPVAVK +++
Sbjct: 257 NKSFHSLCLTDWLRSITTTRQSFDVLFGNCPYCSDPVAVKTSNK 300



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.138    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,017,004
Number of extensions: 365096
Number of successful extensions: 1084
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1082
Number of HSP's successfully gapped: 1
Length of query: 308
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 210
Effective length of database: 8,419,801
Effective search space: 1768158210
Effective search space used: 1768158210
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 111 (47.4 bits)