BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0691400 Os03g0691400|AK071319
         (164 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G48320.1  | chr1:17855024-17855577 REVERSE LENGTH=157          197   2e-51
AT5G48950.1  | chr5:19846200-19847290 FORWARD LENGTH=158          159   6e-40

>AT1G48320.1 | chr1:17855024-17855577 REVERSE LENGTH=157
          Length = 156

 Score =  197 bits (501), Expect = 2e-51,   Method: Compositional matrix adjust.
 Identities = 94/164 (57%), Positives = 115/164 (70%), Gaps = 8/164 (4%)

Query: 1   MDDATSSSSRAPRPKTEELDAALHAMGFEIERVSPAEVTGRLLVTPTCCQPFKVLHGGVS 60
           MD A+S+        T+ +D  LH +GFE + +SP  +TGRL V+P CCQPFKVLHGGVS
Sbjct: 1   MDSASSN--------TKAIDPPLHMLGFEFDELSPTRITGRLPVSPVCCQPFKVLHGGVS 52

Query: 61  ALIAEGLASMGAHMASGYSRVAGVQLSINHFRSAALGDTVLVRAAPLHVGRSTQVWAVKL 120
           ALIAE LASMGAHMASG+ RVAG+QLSINH +SA LGD V   A P+  G++ QVW VKL
Sbjct: 53  ALIAESLASMGAHMASGFKRVAGIQLSINHLKSADLGDLVFAEATPVSTGKTIQVWEVKL 112

Query: 121 WKLDPSTKEKGAQISESRVTLLCNLPVPESVKNAGEALKKYSKL 164
           WK     K     IS SRVTL+CNLP+P++ K+A   LK  +KL
Sbjct: 113 WKTTQKDKANKILISSSRVTLICNLPIPDNAKDAANMLKMVAKL 156



>AT5G48950.1 | chr5:19846200-19847290 FORWARD LENGTH=158
          Length = 157

 Score =  159 bits (402), Expect = 6e-40,   Method: Compositional matrix adjust.
 Identities = 82/155 (52%), Positives = 107/155 (69%), Gaps = 4/155 (2%)

Query: 14  PKTEE--LDAALHAMGFEIERVSPAEVTGRLLVTPTCCQPFKVLHGGVSALIAEGLASMG 71
           PK+ E  +D  L  +GF  + +S   V+G L +T  CCQPFKVLHGGVSALIAE LAS+G
Sbjct: 3   PKSPEFIIDQPLKILGFVFDELSATRVSGHLTLTEKCCQPFKVLHGGVSALIAEALASLG 62

Query: 72  AHMASGYSRVAGVQLSINHFRSAALGDTVLVRAAPLHVGRSTQVWAVKLWKLDPS-TKEK 130
           A +ASG+ RVAG+ LSI+H R AALG+ V   + P+ VG++ QVW V+LWK   + T + 
Sbjct: 63  AGIASGFKRVAGIHLSIHHLRPAALGEIVFAESFPVSVGKNIQVWEVRLWKAKKTETPDN 122

Query: 131 GAQISESRVTLLCNLPVPESVKNAGEALKK-YSKL 164
              +S SRVTL C LP+P+ VK+A + LKK  SKL
Sbjct: 123 KIMVSTSRVTLFCGLPIPDHVKDAPDELKKVISKL 157



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.128    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,328,061
Number of extensions: 114530
Number of successful extensions: 300
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 300
Number of HSP's successfully gapped: 2
Length of query: 164
Length of database: 11,106,569
Length adjustment: 91
Effective length of query: 73
Effective length of database: 8,611,713
Effective search space: 628655049
Effective search space used: 628655049
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 108 (46.2 bits)