BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0650400 Os03g0650400|AK101644
         (803 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G51330.1  | chr5:20858752-20861680 REVERSE LENGTH=640          229   5e-60
AT5G23610.2  | chr5:7957641-7959968 REVERSE LENGTH=501            138   1e-32

>AT5G51330.1 | chr5:20858752-20861680 REVERSE LENGTH=640
          Length = 639

 Score =  229 bits (583), Expect = 5e-60,   Method: Compositional matrix adjust.
 Identities = 191/634 (30%), Positives = 268/634 (42%), Gaps = 92/634 (14%)

Query: 59  SRQSLSSSATHTYHTGGFYEIDHEKLPPKSPIHLKSIRVVKVSGYTSLDVTVXXXXXXXX 118
           S  +L+ +  H    G +YEID   LP +SP +LKSIRVV VS  T+ DV++        
Sbjct: 24  SSPTLNVAVAHI-RAGSYYEIDASILPQRSPENLKSIRVVMVSKITASDVSLRYPSMFSL 82

Query: 119 XXXXXXXXXXCTGPE-----------LDERFVMSSNHAARILRRRVAEEELAGDVMHQDS 167
                        P             DE  VM+S  A  +L RR+A  EL+   M+++S
Sbjct: 83  RSHFDYSRMNRNKPMKKRSGGGLLPVFDESHVMASELAGDLLYRRIAPHELS---MNRNS 139

Query: 168 --FWLVKPCLYDFSASSPHD------VLTXXXXXXXXXXXXXXXXXCLLDTLKCDGAGWG 219
             FW+        S+SS  +      V++                 C  +        WG
Sbjct: 140 WGFWV--------SSSSRRNKFPRREVVSQPAYNTRLCRAASPEGKCSSELKSGGMIKWG 191

Query: 220 VRRRVRYIGRHHDASKEASAASLDGYNTEVSVXXXXXXXXXXXXXXXXXXXXXDNKSTSN 279
            R RV+Y  RH D  K            EV                       + K  +N
Sbjct: 192 RRLRVQYQSRHIDTRKNKEGEESSRVKDEVYKEEEMEKEEDDDDGNEIGGTKQEAKEITN 251

Query: 280 GKRKREEAESSMDXXXXXXXXXXXXXXXXXXXXXXXXXXAKDGDPRRGKDRWSAERYAAA 339
           G RKR+  ESS +                           K    R+  DRWS ERY  A
Sbjct: 252 GNRKRKLIESSTERLAQKAKVYDQKKETQIVVY-------KRKSERKFIDRWSVERYKLA 304

Query: 340 ERSLLDIMRSHGACFGAPVMRQALREEARKHIGDTGLLDHLLKHMAGRVPEGSADRFRRR 399
           ER++L +M+   A FG  ++R  LR EARK IGDTGLLDHLLKHMAG+V  G  DRF R+
Sbjct: 305 ERNMLKVMKEKNAVFGNSILRPELRSEARKLIGDTGLLDHLLKHMAGKVAPGGQDRFMRK 364

Query: 400 HNADGAMEYWLEPAELAEVRRLAGVSDPYWVPPPGWKPGDDVS---AVAGDXXXXXXXXX 456
           HNADGAMEYWLE ++L  +R+ AGV DPYW PPPGWK GD+ S     AG+         
Sbjct: 365 HNADGAMEYWLESSDLIHIRKEAGVKDPYWTPPPGWKLGDNPSQDPVCAGE--------- 415

Query: 457 XXXXXDGVKRHIEQLSSNLVQLEKETKSEAERSYSSRKEKYQKLMKANEKLEKQ----VL 512
                      I  +   L  L++E K  A +     +E+   +   N  +  Q    + 
Sbjct: 416 -----------IRDIREELASLKRELKKLASK---KEEEELVIMTTPNSCVTSQNDNLMT 461

Query: 513 SMKDMYEHLVQKKGKLKKEVLSLKDKYKLVLEKNDKLEEQMASLSSSF--------LSLK 564
             K++Y  L++KK K       ++D+  ++ E   K+EE M  L  +          S +
Sbjct: 462 PAKEIYADLLKKKYK-------IEDQLVIIGETLRKMEEDMGWLKKTVDENYPKKPDSTE 514

Query: 565 EQLLLPRNGDNLNMERERVEVTLGKQEGLVPGEPLYVDGGDRISQQADATVVQVGEKRTA 624
             LLL  +     +E E   V  G Q   +   P   + G +  QQ  + +  +      
Sbjct: 515 TPLLLEDSPPIQTLEGEVKVVNKGNQ---ITESPQNREKGRKHDQQERSPLSLISN---- 567

Query: 625 RKSSFRICKPQGTFMWPHMASGTSMAISGGGSSS 658
             + FRIC+P G F WP + +  +   +   S S
Sbjct: 568 --TGFRICRPVGMFAWPQLPALAAATDTNASSPS 599



>AT5G23610.2 | chr5:7957641-7959968 REVERSE LENGTH=501
          Length = 500

 Score =  138 bits (348), Expect = 1e-32,   Method: Compositional matrix adjust.
 Identities = 85/239 (35%), Positives = 125/239 (52%), Gaps = 35/239 (14%)

Query: 330 RWSAERYAAAERSLLDIMRSHGACFGAPVMRQALREEARKHIGDTGLLDHLLKHMAGRVP 389
           RW+ ER   AE++L DIM+  GA F  PV RQ LR  AR  IGDTGLLDH LKHM G+V 
Sbjct: 124 RWNNERIKFAEQTLADIMKEKGATFEKPVTRQLLRVIARSKIGDTGLLDHSLKHMDGKVT 183

Query: 390 EGSADRFRRRHNADGAMEYWLEPAELAEVRRLAGVSDPYWVPPPGW----KPGDDVSAVA 445
            G +DRFRR +N DG M+YWLE A+L +++  +G+ DP WVPP  W        D SAV 
Sbjct: 184 PGGSDRFRRCYNTDGCMQYWLESADLVKIKLESGIPDPTWVPPAWWMVQTASSHDQSAVT 243

Query: 446 GDXXXXXXXXXXXXXXDGVKRHIEQLSSNLVQLEKETKSEAERSYSSRKEKYQKLMKANE 505
                             +   IEQ+ S + +L               K+       ANE
Sbjct: 244 SKL---------------LMGEIEQMKSEIKEL-------------VSKQNLPDHADANE 275

Query: 506 KLEKQVLSMKDMYEHLVQKKGKLKKEVLSLKDKYKLVLEKNDKLEEQMASLSSSFLSLK 564
           KL K+   +K   E+  ++  ++ + + S +  +K +    DK+++Q+  +S++  +L+
Sbjct: 276 KLFKE---LKSWRENTDKQIVEISESLTSTQGMFKELNSWKDKVDQQLLGISNTLSNLQ 331



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.130    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 14,353,611
Number of extensions: 534042
Number of successful extensions: 2205
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 2213
Number of HSP's successfully gapped: 2
Length of query: 803
Length of database: 11,106,569
Length adjustment: 107
Effective length of query: 696
Effective length of database: 8,173,057
Effective search space: 5688447672
Effective search space used: 5688447672
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 116 (49.3 bits)