BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0644000 Os03g0644000|AK066359
         (501 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G61710.1  | chr3:22839477-22842253 REVERSE LENGTH=518          543   e-154

>AT3G61710.1 | chr3:22839477-22842253 REVERSE LENGTH=518
          Length = 517

 Score =  543 bits (1398), Expect = e-154,   Method: Compositional matrix adjust.
 Identities = 268/487 (55%), Positives = 340/487 (69%), Gaps = 19/487 (3%)

Query: 18  VDPSLPRFKCQECQRALVVVGVESFTDKL----PAHAVSGMNVSSVQG--SVMGASRMDN 71
           +DP+LP++ CQ C  +L +VGV+S+  K     P  A  G   SS+ G  SV+G++RMDN
Sbjct: 15  IDPNLPKWVCQNCHHSLTIVGVDSYAGKFFNDPPPSATQG---SSIHGANSVLGSTRMDN 71

Query: 72  SYVVLSKQNRSHSHGIPPRPPSAGIPRAEPNQPTRAMEGSYIVLPPAAASIYKTS-ASEG 130
           S+VVL +     S GIPPRP  A  P+ +  Q  +AME S++V       +YK+   S+ 
Sbjct: 72  SFVVLPRHKPPQSQGIPPRPRGASSPQPDATQSGKAMEESFVV-------VYKSEPVSDS 124

Query: 131 GGAQLSPTSMNPGSPLPGNN--FHSSVTVLKRAFEIATSQTQIEQPLCLDCMRLLSDKME 188
           GG+      +    PL  N   F++++ VL RAF+IA +QTQ+EQPLCL+CMR+LSDK+E
Sbjct: 125 GGSHNLSLEVGQNGPLHSNTSGFNATINVLTRAFDIARTQTQVEQPLCLECMRVLSDKLE 184

Query: 189 KEIEDVNTDNKAYEACLQRLEQETYNILSETDFXXXXXXXXXXXXXXXXXXXXXXXXYSE 248
           KE+EDV  D +AYEAC+QRLE ET ++LSE DF                         +E
Sbjct: 185 KEVEDVTRDVEAYEACVQRLEGETQDVLSEADFLKEKKKIEEEERKLVAAIEETEKQNAE 244

Query: 249 ICSEMKCLETKSKQFEELEERYCHDLNSFQFQWISHQEERDAVLAKIEVSQVHLELLKRT 308
           +  ++K LE K  +F ELE+RY  + N+FQFQ I+HQEERDA+LAKIEVSQ HLELL +T
Sbjct: 245 VNHQLKELEFKGNRFNELEDRYWQEFNNFQFQLIAHQEERDAILAKIEVSQAHLELLNKT 304

Query: 309 NVLNDAFYISHDGVIGTINNFRLGRLPNVQVEWDEINAAWGQAALLLHTMAQYFFPKFEY 368
           NVL DAF I +DG  GTINNFRLGRLP ++VEWDEINAAWGQA LLLHTM  YF PKF+ 
Sbjct: 305 NVLIDAFPIRNDGEFGTINNFRLGRLPAIKVEWDEINAAWGQACLLLHTMCNYFRPKFQC 364

Query: 369 RIKIHPMGSYPKVTDINQNTYELFGPVNLFWSTRFDKAMTWFLTCLQEFADFAVSLDKEN 428
           ++KI PMGSYP++ D N  TYELFGPVNLFWSTR+DKAMT +L CL++FADFA S D+EN
Sbjct: 365 QVKIQPMGSYPRIVDSNNETYELFGPVNLFWSTRYDKAMTLYLMCLKDFADFANSKDQEN 424

Query: 429 NVPPDKSLKLPYKIEGDKVGSYTIFLSFNKLDNWTKALKYMLCNLKWVLYWFIGNTSFAP 488
           N+PPD  L LPYKIE DKV  Y+I  SFNK ++WTKALKY LCNLKW LYWF+GNT+F P
Sbjct: 425 NIPPDNCLNLPYKIEKDKVLGYSITQSFNKQESWTKALKYTLCNLKWALYWFVGNTNFQP 484

Query: 489 PSGSLHV 495
            S ++ +
Sbjct: 485 LSATVSL 491



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.317    0.133    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 10,837,261
Number of extensions: 456150
Number of successful extensions: 1427
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1424
Number of HSP's successfully gapped: 1
Length of query: 501
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 398
Effective length of database: 8,282,721
Effective search space: 3296522958
Effective search space used: 3296522958
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 114 (48.5 bits)