BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0639300 Os03g0639300|AK060505
         (307 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G20180.2  | chr2:8704525-8706538 REVERSE LENGTH=479             58   8e-09

>AT2G20180.2 | chr2:8704525-8706538 REVERSE LENGTH=479
          Length = 478

 Score = 57.8 bits (138), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 38/113 (33%), Positives = 49/113 (43%), Gaps = 31/113 (27%)

Query: 1   MNQFVPDWSNMGD---------------ASRTLGEDDNLIELLWCNGHVVMQSQNHHRKL 45
           M+ FVPD+    D               +  T+GEDD+L+ELLW NG VV+Q+Q  H K 
Sbjct: 1   MHHFVPDFDTDDDYVNNHNSSLNHLPRKSITTMGEDDDLMELLWQNGQVVVQNQRLHTKK 60

Query: 46  PPRPPEKX-------------XXXXVQEDEAGLWFPFALADSLEKDIFSDLFY 85
           P   P K                  +QEDE   W  + L D    D  SDL +
Sbjct: 61  PSSSPPKLLPSMDPQQQPSSDQNLFIQEDEMTSWLHYPLRDD---DFCSDLLF 110



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.311    0.129    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,606,321
Number of extensions: 176002
Number of successful extensions: 330
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 330
Number of HSP's successfully gapped: 1
Length of query: 307
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 209
Effective length of database: 8,419,801
Effective search space: 1759738409
Effective search space used: 1759738409
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 111 (47.4 bits)