BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0612600 Os03g0612600|AK071403
(66 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G66860.1 | chr5:26701233-26702531 REVERSE LENGTH=250 89 5e-19
AT4G23620.2 | chr4:12315016-12316659 REVERSE LENGTH=278 63 4e-11
>AT5G66860.1 | chr5:26701233-26702531 REVERSE LENGTH=250
Length = 249
Score = 88.6 bits (218), Expect = 5e-19, Method: Composition-based stats.
Identities = 42/65 (64%), Positives = 54/65 (83%)
Query: 2 KKIRTSLVYLCPAEHIPPKIEVDLANLDIGDRVSMNDIPVHPSLRLLSKNETMPVCKILA 61
+ IR++L L PAEHIP KIEVD++NLDI D+V + D+ HPSL+LLSKNETMPVCKI+A
Sbjct: 178 RTIRSTLKLLGPAEHIPSKIEVDVSNLDIEDKVLLQDVVFHPSLKLLSKNETMPVCKIVA 237
Query: 62 SKPVE 66
+ PV+
Sbjct: 238 TSPVK 242
>AT4G23620.2 | chr4:12315016-12316659 REVERSE LENGTH=278
Length = 277
Score = 62.8 bits (151), Expect = 4e-11, Method: Compositional matrix adjust.
Identities = 28/60 (46%), Positives = 44/60 (73%), Gaps = 1/60 (1%)
Query: 4 IRTSLVYLCPAEHIPPKIEVDLANLDIGDRVSMNDIPVHPSLRLLSKNETMPVCKILASK 63
I+ ++ +LCPAE IPP IEVDL+ LDIG ++ M D+ VHP+L+L+ K++ P+ K+ +
Sbjct: 205 IKRTVKFLCPAEIIPPYIEVDLSQLDIGQKLVMGDLKVHPALKLI-KSKDEPIVKVAGGR 263
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.319 0.137 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,410,813
Number of extensions: 43268
Number of successful extensions: 97
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 97
Number of HSP's successfully gapped: 2
Length of query: 66
Length of database: 11,106,569
Length adjustment: 39
Effective length of query: 27
Effective length of database: 10,037,345
Effective search space: 271008315
Effective search space used: 271008315
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 104 (44.7 bits)