BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0587000 Os03g0587000|AK071149
         (267 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G02870.1  | chr3:627742-629682 REVERSE LENGTH=272              369   e-103
AT1G31190.1  | chr1:11144861-11146800 FORWARD LENGTH=372          144   3e-35
AT4G39120.1  | chr4:18225578-18227988 REVERSE LENGTH=376           87   1e-17

>AT3G02870.1 | chr3:627742-629682 REVERSE LENGTH=272
          Length = 271

 Score =  369 bits (948), Expect = e-103,   Method: Compositional matrix adjust.
 Identities = 180/260 (69%), Positives = 217/260 (83%), Gaps = 1/260 (0%)

Query: 4   EQFLAVAVDAAKNAGEIIRKGFYQTKNVEHKGQVDLVTETDKACEDLIFNHLRKHYPDHK 63
           +QFLA A+DAAK AG+IIRKGFY+TK+VEHKGQVDLVTETDK CE+L+FNHL++ +P+HK
Sbjct: 8   DQFLAAAIDAAKKAGQIIRKGFYETKHVEHKGQVDLVTETDKGCEELVFNHLKQLFPNHK 67

Query: 64  FIGEEXXXXXXXXXXXXXXXXWIVDPLDGTTNFVHGFPFVCVSIGLTVGKIPTVGVVYNP 123
           FIGEE                WIVDPLDGTTNFVHGFPFVCVSIGLT+GK+P VGVVYNP
Sbjct: 68  FIGEETTAAFGVTELTDEPT-WIVDPLDGTTNFVHGFPFVCVSIGLTIGKVPVVGVVYNP 126

Query: 124 IMNELFTAVRGKGAFLNGSPIKTSSQNELVKALLVTEVGTKRDKATLDDTTNRINKLLFK 183
           IM ELFT V+GKGAFLNG  IK S+Q+EL+ ALLVTE GTKRDKATLDDTTNRIN LL K
Sbjct: 127 IMEELFTGVQGKGAFLNGKRIKVSAQSELLTALLVTEAGTKRDKATLDDTTNRINSLLTK 186

Query: 184 IRSIRMCGSLALNMCGVACGRLDLCYEIGFGGPWDVAAGALILREAGGFVFDPSGGEFDL 243
           +RS+RM GS AL++CGVACGR+D+ YE+GFGGPWD+AAG +I++EAGG +FDPSG + D+
Sbjct: 187 VRSLRMSGSCALDLCGVACGRVDIFYELGFGGPWDIAAGIVIVKEAGGLIFDPSGKDLDI 246

Query: 244 MARRMAGSNSYLKDQFIKEL 263
            ++R+A SN+ LK+ F + L
Sbjct: 247 TSQRIAASNASLKELFAEAL 266



>AT1G31190.1 | chr1:11144861-11146800 FORWARD LENGTH=372
          Length = 371

 Score =  144 bits (364), Expect = 3e-35,   Method: Compositional matrix adjust.
 Identities = 93/275 (33%), Positives = 135/275 (49%), Gaps = 22/275 (8%)

Query: 1   MAEEQFLAVAVDAAKNAGEIIRKGFYQTKNVEHKGQVDLVTETDKACEDLIFNHLRKHYP 60
           ++    L V   AAK   E++ +   + +N+ +KG  DLVT+TDKA E  I   ++K++ 
Sbjct: 82  ISPAHLLEVVELAAKTGAEVVMEAVNKPRNITYKGLSDLVTDTDKASEAAILEVVKKNFS 141

Query: 61  DHKFIGEEXXXXXXXXXXXXXXXXWIVDPLDGTTNFVHGFPFVCVSIGLTVGKIPTVGVV 120
           DH  +GEE                W +DPLDGTTNF HG+P   VS+G+     P    V
Sbjct: 142 DHLILGEE----GGIIGDSSSDYLWCIDPLDGTTNFAHGYPSFAVSVGVLYRGNPAAASV 197

Query: 121 YNPIMNEL------FTAVRGKGAFLNGSPIKTSSQNELVKALLVTEVGTKRDKATLDDTT 174
              +   +      F+A  G GA  NG  I  S  + + +ALL+T  G + D A      
Sbjct: 198 VEFVGGPMCWNTRTFSATAGGGALCNGQKIHVSKTDAVERALLITGFGYEHDDAW----- 252

Query: 175 NRINKLLFK-----IRSIRMCGSLALNMCGVACGRLDLCYEIGFGGPWDVAAGALILREA 229
              N  LFK      R +R  G+ A++MC VA G  +  +E     PWD+AAG LI+ EA
Sbjct: 253 -STNMELFKEFTDVSRGVRRLGAAAVDMCHVALGIAESYWEYRL-KPWDMAAGVLIVEEA 310

Query: 230 GGFVFDPSGGEFDLMARRMAGSNSYLKDQFIKELG 264
           GG V    GG+F +  R +  SN  L  + ++ + 
Sbjct: 311 GGAVTRMDGGKFSVFDRSVLVSNGVLHPKLLERIA 345



>AT4G39120.1 | chr4:18225578-18227988 REVERSE LENGTH=376
          Length = 375

 Score = 87.0 bits (214), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 69/254 (27%), Positives = 116/254 (45%), Gaps = 15/254 (5%)

Query: 4   EQFLAVAVDAAKNAGEIIRKGFYQTKNVEHKGQVDLVTETDKACEDLIFNHLRKHYPDHK 63
           ++F AV    A  +GE+IRK F +  ++  K  +  VT  D+  E+ + + + ++ P H 
Sbjct: 113 DRFAAVGNALADASGEVIRKYFRKKFDIVDKDDMSPVTIADQMAEEAMVSIIFQNLPSHA 172

Query: 64  FIGEEXXXXXXXXXXXXXXXXWIVDPLDGTTNFVHGFPFVCVSIGLTVGKIPTVGVVYNP 123
             GEE                W++DP+DGT +F+ G P     I L     P +G++  P
Sbjct: 173 IYGEEKGWRCKEESADYV---WVLDPIDGTKSFITGKPVFGTLIALLYKGKPILGLIDQP 229

Query: 124 IMNELFTAVRGKGAFLNGSPIKTSSQNELVKALLVT---EVGTKRDKATLDDTTNRINKL 180
           I+ E +  + G+   LNG  I T S  +L +A L T    + ++  +       +++   
Sbjct: 230 ILKERWIGMNGRRTKLNGEDISTRSCPKLSQAYLYTTSPHLFSEEAEKAYSRVRDKVKVP 289

Query: 181 LFKIRSIRMCGSLALNMCGVACGRLDLCYEIGFGGPWDVAAGALILREAGGFVFDPSGGE 240
           L+       C + AL    +A G +DL  E G   P+D  A   ++  AGG + D +G  
Sbjct: 290 LYGCD----CYAYAL----LASGFVDLVIESGL-KPYDFLALVPVIEGAGGTITDWTGKR 340

Query: 241 FDLMARRMAGSNSY 254
           F   A   A + S+
Sbjct: 341 FLWEASSSAVATSF 354



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.140    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,451,717
Number of extensions: 217913
Number of successful extensions: 433
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 427
Number of HSP's successfully gapped: 3
Length of query: 267
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 170
Effective length of database: 8,447,217
Effective search space: 1436026890
Effective search space used: 1436026890
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 111 (47.4 bits)