BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0586600 Os03g0586600|AK070943
         (142 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G51940.1  | chr5:21104679-21105796 FORWARD LENGTH=145          188   8e-49
AT2G04630.1  | chr2:1619168-1620246 REVERSE LENGTH=145            187   2e-48

>AT5G51940.1 | chr5:21104679-21105796 FORWARD LENGTH=145
          Length = 144

 Score =  188 bits (477), Expect = 8e-49,   Method: Compositional matrix adjust.
 Identities = 101/143 (70%), Positives = 107/143 (74%), Gaps = 2/143 (1%)

Query: 1   MADDDYNDMD-MGYXXXXXXXXXXXXXXXXXXXXXXXXXXVVGAEAEDK-EQEKTQRPRK 58
           MAD+DYND+D +GY                              EAEDK E E  QRPRK
Sbjct: 1   MADEDYNDVDDLGYEDEPAEPEIEEGVEEDVEMKENDDVNGEPIEAEDKVETEPVQRPRK 60

Query: 59  TSKYMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRARKIPFTIRRYLPD 118
           TSK+MTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELR RKIPFTIRRYLPD
Sbjct: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRQRKIPFTIRRYLPD 120

Query: 119 GSYEDWGVDELIVEDSWKRQVGG 141
           GS+E+WGVDELIVEDSWKRQVGG
Sbjct: 121 GSFEEWGVDELIVEDSWKRQVGG 143



>AT2G04630.1 | chr2:1619168-1620246 REVERSE LENGTH=145
          Length = 144

 Score =  187 bits (474), Expect = 2e-48,   Method: Compositional matrix adjust.
 Identities = 101/143 (70%), Positives = 106/143 (74%), Gaps = 2/143 (1%)

Query: 1   MADDDYNDMD-MGYXXXXXXXXXXXXXXXXXXXXXXXXXXVVGAEAEDK-EQEKTQRPRK 58
           MADDDYN++D +GY                          V   E EDK E E  QRPRK
Sbjct: 1   MADDDYNEVDDLGYEDEPAEPEIEEGVEEDADIKENDDVNVDPLETEDKVETEPVQRPRK 60

Query: 59  TSKYMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRARKIPFTIRRYLPD 118
           TSK+MTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELR RKIPFTIRRYLPD
Sbjct: 61  TSKFMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRQRKIPFTIRRYLPD 120

Query: 119 GSYEDWGVDELIVEDSWKRQVGG 141
            SYE+WGVDELIVEDSWKRQVGG
Sbjct: 121 MSYEEWGVDELIVEDSWKRQVGG 143



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.134    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,533,736
Number of extensions: 80678
Number of successful extensions: 176
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 174
Number of HSP's successfully gapped: 2
Length of query: 142
Length of database: 11,106,569
Length adjustment: 89
Effective length of query: 53
Effective length of database: 8,666,545
Effective search space: 459326885
Effective search space used: 459326885
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 106 (45.4 bits)