BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0586500 Os03g0586500|AK068097
(271 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G15840.1 | chr3:5356782-5358418 REVERSE LENGTH=269 306 6e-84
>AT3G15840.1 | chr3:5356782-5358418 REVERSE LENGTH=269
Length = 268
Score = 306 bits (785), Expect = 6e-84, Method: Compositional matrix adjust.
Identities = 141/211 (66%), Positives = 172/211 (81%), Gaps = 2/211 (0%)
Query: 63 QTNECSLPTWAEFELGKAPVYWKTMNGLPPSAGEGLILFYNPAATKMTPNAQFGIAFNGG 122
+ E LP+WA FE+G APVYWKTMNGLPP++GE L LFYNPAA+K+T N +G+AFNGG
Sbjct: 58 EIKEYKLPSWAMFEMGTAPVYWKTMNGLPPTSGEKLKLFYNPAASKLTLNEDYGVAFNGG 117
Query: 123 FNQPIMCGGEPRQMTLQERGSADPPIYTIRIRVPQHAMTLVFSFTNGVDWDGPYTLKFRV 182
FNQPIMCGGEPR M ++RG AD PIYT++I +P+HA+ L+FSFTNGVDWDGPY L+F+V
Sbjct: 118 FNQPIMCGGEPRAMLKKDRGKADSPIYTMQICIPKHAVNLIFSFTNGVDWDGPYRLQFQV 177
Query: 183 PKPWLNKPLSFFNEGLADELNREGACDRAIFPDENVVITSCEM-GSYYEEGGDRCKLDIV 241
PK W NKP+ FFNEGLA+EL+++GAC+RAIFPD NVV T C M + EGGDRC LD+V
Sbjct: 178 PKRWQNKPIEFFNEGLANELSQDGACERAIFPDSNVVPTRCTMIANLTVEGGDRCNLDLV 237
Query: 242 SGCMDPNSHMFDPLATVDDGSCPMD-SDSEE 271
GCMD NS F+P A VDDGSCP++ SDS+E
Sbjct: 238 PGCMDTNSEHFNPYANVDDGSCPLELSDSDE 268
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.318 0.136 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,081,614
Number of extensions: 253554
Number of successful extensions: 421
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 420
Number of HSP's successfully gapped: 1
Length of query: 271
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 174
Effective length of database: 8,447,217
Effective search space: 1469815758
Effective search space used: 1469815758
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 111 (47.4 bits)