BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0577100 Os03g0577100|AK101786
         (482 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G61000.1  | chr1:22471096-22474046 REVERSE LENGTH=441          224   1e-58

>AT1G61000.1 | chr1:22471096-22474046 REVERSE LENGTH=441
          Length = 440

 Score =  224 bits (570), Expect = 1e-58,   Method: Compositional matrix adjust.
 Identities = 147/445 (33%), Positives = 245/445 (55%), Gaps = 14/445 (3%)

Query: 3   SNFSFPPLAPEQIAEALHTYGLAPTANLRAEDIANPQPDLLPAVISNFLATVVDPTGADD 62
           S + +P L+   I  AL     A  A++   D+  P  D +  + +  L  +      D 
Sbjct: 2   SAYEYPRLSRSDIITALKD---AQIASVTETDLKTPTSDFVSELYTRILIYL------DA 52

Query: 63  LD----GQLGFDALASLDNPEHYREGIRVLRLHKRANAFLESIQFPGFTXXXXXXXXXXX 118
           LD    GQ+ F+AL  L+NP+H+   ++ ++L+ +    LE +  P              
Sbjct: 53  LDEEEKGQVDFEALEQLENPDHHATSMQAMKLYCKVKDMLEMLDCPLPISFKDLLRPESS 112

Query: 119 XVQV-LSALINFLYYRDEKLALLQPIIHEFPNLDERCMELNAKIAEHQKAIADQELAAQM 177
             +  +SAL+N+  Y+D K+ L++P   E   LDE+  +  AK+A+    I + + A + 
Sbjct: 113 RTEFFISALLNYGLYKDSKMDLIRPKAEELGLLDEQRKQCEAKVAQLNAEIGEFDEAVER 172

Query: 178 EVPMVQQLEAEVNSLKQKLVEYNKKQLALRANATAINDKKEETHRKIAKSDFELVKLAQE 237
           ++P VQ+LEA +  L +K++E N +Q++LRA    + +K  +   +I+K++F+LV+  QE
Sbjct: 173 DLPFVQELEANIEQLNKKILELNNQQMSLRATFQKMREKSTQMDNEISKAEFDLVETVQE 232

Query: 238 NSKLLSKIVQSPEKLQRALEEKKTARAELKNAEKIAMQSVQEKTATLEIYSKGYEKLSKH 297
           N+ L S+IVQSP+KLQ ALEEKK    E K AE+ AM + QEK A LE++ K  +K+ K 
Sbjct: 233 NANLRSQIVQSPDKLQGALEEKKLVLGETKKAEQSAMVTFQEKAAILEVFEKALKKILKS 292

Query: 298 SSKIQALQEQFTATKALEKEVKARKAKISDESVEIMALDTKTIEWDGKVHEMEERVKAKE 357
           SS++Q + EQ T  K +EKE KA K K+S++ V   +L+ K +E +  V ++ E +K  E
Sbjct: 293 SSQLQLINEQVTNAKTVEKEFKALKDKLSEDGVAYKSLEAKVVERERIVEQLNESLKQLE 352

Query: 358 KERDQIVADENQKLAALRSEVEWKLKCLEPRERKVEETIAKATKLCAEVDSTRTDAAEEL 417
           KE+  +  D  ++L  L+ EVE + + LE R+  VE  +A      A+ +  R     ++
Sbjct: 353 KEKAVMFDDWTKQLNELKVEVESRRRELETRQTNVESVVAMVDDNTAKTNQVRQSGEAKV 412

Query: 418 RLIYAKFQQIGHAFTCYKDNFKTFL 442
           + + AK+++I   F  Y  +F  FL
Sbjct: 413 KKLAAKYEEIVKQFHEYTVSFDAFL 437



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.312    0.127    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 9,031,475
Number of extensions: 349820
Number of successful extensions: 2371
Number of sequences better than 1.0e-05: 39
Number of HSP's gapped: 2410
Number of HSP's successfully gapped: 59
Length of query: 482
Length of database: 11,106,569
Length adjustment: 102
Effective length of query: 380
Effective length of database: 8,310,137
Effective search space: 3157852060
Effective search space used: 3157852060
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 114 (48.5 bits)