BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0452300 Os03g0452300|AK065820
         (326 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G33800.1  | chr2:14300925-14302352 REVERSE LENGTH=304          235   2e-62
AT1G64880.1  | chr1:24105713-24108780 FORWARD LENGTH=516           49   3e-06

>AT2G33800.1 | chr2:14300925-14302352 REVERSE LENGTH=304
          Length = 303

 Score =  235 bits (600), Expect = 2e-62,   Method: Compositional matrix adjust.
 Identities = 122/246 (49%), Positives = 155/246 (63%), Gaps = 11/246 (4%)

Query: 89  EKPRPEPVAASGFEFASPPEGYVEPAPFDELPPESPEDVAAAYEALYGPAFSGETVMGNN 148
           E   PE  A   F+    PEG+V P  FDE   E+ E++A A+E LYGPA+SGE+++G +
Sbjct: 61  EDETPEITANVVFDPPIAPEGFVSPPYFDEGSDETEEEIATAFEELYGPAYSGESMLGKD 120

Query: 149 VFEVKVVDPVDMD--------REQRPSDEFSEXXXXXXXXXXXXXGGRQLSFRAIXXXXX 200
           ++   V+D             ++ +  D F E             GG+QL FRAI     
Sbjct: 121 IY---VMDSKHKKSSGIGGKPKKDKIRDGFEERVVQVRRVTKVVKGGKQLKFRAIVVVGD 177

Query: 201 XXXXXXXXXXXXXXXTEAITKAAMNGRRNLVTVPLTKYSTFPHRADADYGAARVMLRPAC 260
                            A+ K+A++ RRN+V VP+TKYSTFPHR++ DYGAA+VMLRPA 
Sbjct: 178 KQGNVGVGCAKAKEVVAAVQKSAIDARRNIVQVPMTKYSTFPHRSEGDYGAAKVMLRPAS 237

Query: 261 PGSGVIAGGAVRVVLEMAGVENALGKQLRSKNPLNNARATIKATQMMRQFKDVAAERGLP 320
           PG+GVIAGGAVR+VLEMAGVENALGKQL S N LNNARAT+ A Q MRQF+DVA ERG+P
Sbjct: 238 PGTGVIAGGAVRIVLEMAGVENALGKQLGSNNALNNARATLAAVQQMRQFRDVAQERGIP 297

Query: 321 MEQLWK 326
           ME+LWK
Sbjct: 298 MEELWK 303



>AT1G64880.1 | chr1:24105713-24108780 FORWARD LENGTH=516
          Length = 515

 Score = 49.3 bits (116), Expect = 3e-06,   Method: Compositional matrix adjust.
 Identities = 31/101 (30%), Positives = 48/101 (47%)

Query: 218 AITKAAMNGRRNLVTVPLTKYSTFPHRADADYGAARVMLRPACPGSGVIAGGAVRVVLEM 277
           A+ KA     +NL  V   +  T  H     Y   ++ L PA   +G+ AG  V+ +L +
Sbjct: 406 AMQKAYEKCFQNLHYVERHEEHTIAHAIQTSYKKTKLYLWPAPTTTGMKAGRVVKTILLL 465

Query: 278 AGVENALGKQLRSKNPLNNARATIKATQMMRQFKDVAAERG 318
           AG +N   K + S+N  N  +A +KA   +   KDV  + G
Sbjct: 466 AGFKNIKSKVIGSRNSYNTVKAVLKALNAVETPKDVQEKFG 506



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.132    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,967,801
Number of extensions: 190789
Number of successful extensions: 713
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 711
Number of HSP's successfully gapped: 2
Length of query: 326
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 227
Effective length of database: 8,392,385
Effective search space: 1905071395
Effective search space used: 1905071395
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 112 (47.8 bits)