BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0424600 Os03g0424600|AK101882
         (544 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G42170.1  | chr3:14321838-14323928 FORWARD LENGTH=697          255   4e-68
AT1G18560.1  | chr1:6385614-6388005 FORWARD LENGTH=691             90   3e-18

>AT3G42170.1 | chr3:14321838-14323928 FORWARD LENGTH=697
          Length = 696

 Score =  255 bits (651), Expect = 4e-68,   Method: Compositional matrix adjust.
 Identities = 144/450 (32%), Positives = 226/450 (50%), Gaps = 18/450 (4%)

Query: 1   MIVLQELPSSFVEHLGFRRFCASLNPYFRVVSRTTIKGDCLAAYQEQKLALLEVLRKTNS 60
           MI++ + P   V+H GF  F  S+ P+F  VS   ++GDC+A Y  +K  +++ L     
Sbjct: 172 MIIMHDYPLHMVQHPGFVSFVQSIQPHFDAVSFNNVQGDCVATYLAEKQNVMKSLEGIPG 231

Query: 61  RVSLTADMWSSNQNLGYMCITCHFIDNEWNMQKRIIRFTQVRTPHDSIALFNEIIKCIQD 120
           R  LT D W+S   LGY+ IT H+ID++W +QK+++       P    AL   +  C+ +
Sbjct: 232 RFCLTLDFWTSKLTLGYVFITAHYIDSDWKIQKKLLNVLMESYPEADEALSLAVANCVSE 291

Query: 121 WQIESKLFSITVDNASTNDSMVVELKGNLLGKLAMPCKGELFHFWCAAHIFNPIVQDGLS 180
           W +E KLF++T ++ ++N S V  ++  L  K      G+L    C A  F  + +D L 
Sbjct: 292 WGLEGKLFNVTFNHPASN-SAVENIRPQLCIKNPGILDGQLVIGNCVARTFGSLAKDVLE 350

Query: 181 TISAAISRIRESVKYVRASEAREQKFEEIIAQVGIVEEKRPSLDVSTRWNSTYLMLSSSL 240
                I  IR+SVK+V+ SE+ E++F E+  Q+ +  EK  SLD  T+WN+TY+ML ++ 
Sbjct: 351 KGKDVIKNIRDSVKHVKTSESHEERFTELKEQLQVPSEKVLSLDDQTQWNTTYMMLVAAS 410

Query: 241 LFRRAFDSLDRFDPNFKDAPQFFDWENAEVLCKHLKVFYDATVVVSGTSYPTVTRYFHEF 300
             +  F  LD  DP++K  P   DW + E LC  LK  ++A   +  T  P+   +FHE 
Sbjct: 411 ELKEVFSCLDTADPDYKKPPSAEDWRHVEALCTFLKPLFEAVSTLQSTGNPSAVTFFHEV 470

Query: 301 WKVKVMILNESNNEDDLVASLVSKMDTKFQKYWDLQFLQIWVPVIFDPRFKLKFLEFRLK 360
           WK +  +      ED  V  +   M  K  KYW    L + + V+ DPRFK+K +EF   
Sbjct: 471 WKTQSDLSRAIAGEDPFVTGIAKTMQEKVDKYWRDCSLVLAMAVVMDPRFKMKLVEFSFS 530

Query: 361 AGFGDKATGPNGYLPRIKRTVRTWFAEYSSQICGPSTTNSQEA-------------STSG 407
             FG+ A G N  +  +   +   F EY + +  P  T S+                T+G
Sbjct: 531 KIFGEDA-GKN--IKTVDDGIHELFTEYMA-LPSPQNTTSEGGKADGLSDFDTYIMETTG 586

Query: 408 VNLHQSLHFLLDKESLATIGVGWLVELWRH 437
            NL   L   LD+  L  +    +++ W+ 
Sbjct: 587 QNLKSELDQYLDETLLPRVQEFDVLDWWKQ 616



>AT1G18560.1 | chr1:6385614-6388005 FORWARD LENGTH=691
          Length = 690

 Score = 89.7 bits (221), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 61/250 (24%), Positives = 111/250 (44%), Gaps = 5/250 (2%)

Query: 2   IVLQELPSSFVEHLGFRRFCASLNPYFRVVSRTTIKGDCLAAYQEQKLALLEVLRKTNSR 61
           + L  LP S V+          L P  ++      K      +   +  +   L    S+
Sbjct: 145 LALSSLPPSTVDETWLGNSFKFLKPSIQLWPAEKYKAILDEVFTSMRGDVKTTLEHIQSK 204

Query: 62  VSLTADMWSSNQNLGYMCITCHFIDNEWNMQKRIIRFTQVRTPHDSIALFNEIIKCIQDW 121
           VS+T   W+S +N+ YM +T  +ID  W+  + ++   ++  P     ++N ++K ++ +
Sbjct: 205 VSVTLSFWNSYENIFYMSVTGQWIDENWSSHRLLLDICRIPYPSGGSEIYNSLLKVLKTY 264

Query: 122 QIESKLFSITVDNASTNDSMVVELKGNLLGKLAMPCKGELFHFWCAAHIFNPIVQDGLST 181
            IE ++   T DN+         LK    G+  +P      +  CAA   N I+ +GL+T
Sbjct: 265 AIEDRILCCTHDNSENAIHACHSLKEYFDGQKVLP----FCYIPCAAQTLNDIIDEGLAT 320

Query: 182 ISAAISRIRESVKYVRASEAREQKFEEIIAQVGIVEEKRPSLDVSTRWNSTYLMLSSSLL 241
           I   IS++RE  + + AS      F ++         K P +D S+RW+  Y M++    
Sbjct: 321 IKPIISKVREFTQELNASTELSDDFIQLTTAYQEGNWKLP-IDASSRWSGNYQMVNILCK 379

Query: 242 FRRAFDSLDR 251
             ++ DS+ R
Sbjct: 380 ASKSLDSVIR 389



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.326    0.138    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 11,717,107
Number of extensions: 471266
Number of successful extensions: 1467
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 1464
Number of HSP's successfully gapped: 2
Length of query: 544
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 441
Effective length of database: 8,282,721
Effective search space: 3652679961
Effective search space used: 3652679961
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 114 (48.5 bits)