BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0353400 Os03g0353400|AK062176
         (160 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G41430.1  | chr2:17269736-17270314 FORWARD LENGTH=164           82   1e-16
AT4G14270.1  | chr4:8218517-8219037 FORWARD LENGTH=144             71   3e-13

>AT2G41430.1 | chr2:17269736-17270314 FORWARD LENGTH=164
          Length = 163

 Score = 82.4 bits (202), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 56/167 (33%), Positives = 74/167 (44%), Gaps = 13/167 (7%)

Query: 1   MSATAVSSLNPDAPLFIPAAFRQVEDFSPQWWDLVKTTAWFRDHWFHEHQQ-----LDEM 55
           M +   S+LNPDAPLFIPAA RQVEDFSP+WW LV T+ W+ D+W  + QQ      D  
Sbjct: 3   MVSGRRSTLNPDAPLFIPAAVRQVEDFSPEWWQLVTTSTWYPDYWISQQQQGADGFYDNG 62

Query: 56  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQTHN--LLVDPPQPPAALKTDAVL 113
                                               Q  +  +    P      K   ++
Sbjct: 63  ENENGGGHIDVADLLPESFDFDDMEDFFDTDAAEFDQGFDGRMYYQAPSEFGFGKNGEMV 122

Query: 114 KALSLVSPKGGDAPRGLREKPRHSEKPTKYAGSPRSGGAPRVIHQPR 160
           K  S     G  +PR + E  +++EKP K+ G+ R   APR IHQPR
Sbjct: 123 KKSS-----GNRSPRSIVEPAKYAEKPAKW-GNQRVAAAPRNIHQPR 163



>AT4G14270.1 | chr4:8218517-8219037 FORWARD LENGTH=144
          Length = 143

 Score = 70.9 bits (172), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 30/54 (55%), Positives = 40/54 (74%), Gaps = 3/54 (5%)

Query: 1  MSATA---VSSLNPDAPLFIPAAFRQVEDFSPQWWDLVKTTAWFRDHWFHEHQQ 51
          M+AT    +S+LNP+AP+F P  FR+VEDFSP+WWDLV T+ WFRD W   + +
Sbjct: 1  MTATIDRRLSTLNPNAPVFDPVEFREVEDFSPKWWDLVTTSKWFRDFWLSANSE 54



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.317    0.132    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,103,082
Number of extensions: 107050
Number of successful extensions: 248
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 248
Number of HSP's successfully gapped: 2
Length of query: 160
Length of database: 11,106,569
Length adjustment: 91
Effective length of query: 69
Effective length of database: 8,611,713
Effective search space: 594208197
Effective search space used: 594208197
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 107 (45.8 bits)