BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0328400 Os03g0328400|AF358764
(44 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G18280.2 | chr5:6050799-6054875 REVERSE LENGTH=631 66 3e-12
AT3G04080.1 | chr3:1068068-1070917 REVERSE LENGTH=472 65 9e-12
>AT5G18280.2 | chr5:6050799-6054875 REVERSE LENGTH=631
Length = 630
Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust.
Identities = 31/43 (72%), Positives = 35/43 (81%)
Query: 1 TLLVDGFGVDPYQDITLVKKVPYSNSFVEAAWPLGSAIEVASS 43
TLL+DGFG++P Q ITLVKKV Y + VEAAWPLGSAIE SS
Sbjct: 587 TLLIDGFGLEPSQTITLVKKVKYGDQAVEAAWPLGSAIEAVSS 629
>AT3G04080.1 | chr3:1068068-1070917 REVERSE LENGTH=472
Length = 471
Score = 64.7 bits (156), Expect = 9e-12, Method: Compositional matrix adjust.
Identities = 32/43 (74%), Positives = 34/43 (79%)
Query: 1 TLLVDGFGVDPYQDITLVKKVPYSNSFVEAAWPLGSAIEVASS 43
TLLVDGFG+ P Q ITLVKKV Y + VEAAWPLGSAIE SS
Sbjct: 428 TLLVDGFGLKPSQTITLVKKVKYGDYAVEAAWPLGSAIEAVSS 470
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.314 0.132 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 879,925
Number of extensions: 20028
Number of successful extensions: 32
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 2
Length of query: 44
Length of database: 11,106,569
Length adjustment: 18
Effective length of query: 26
Effective length of database: 10,613,081
Effective search space: 275940106
Effective search space used: 275940106
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 104 (44.7 bits)