BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0322600 Os03g0322600|AK067222
         (315 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G22340.2  | chr5:7394509-7396609 FORWARD LENGTH=341            180   7e-46

>AT5G22340.2 | chr5:7394509-7396609 FORWARD LENGTH=341
          Length = 340

 Score =  180 bits (457), Expect = 7e-46,   Method: Compositional matrix adjust.
 Identities = 101/248 (40%), Positives = 150/248 (60%), Gaps = 15/248 (6%)

Query: 50  EEVLRMFLAERQAHGDFVTKISDMVWRRNGGDLGVLEAAAEQENSADVAPPQPEE----- 104
           E+VL+MF+ +R+ +GDF++K+SD +W +      +LE+     N A  +  + ++     
Sbjct: 57  EDVLQMFMKDREENGDFISKVSDRLWLKE-----ILESIDVNSNGASSSAVELDDTRESL 111

Query: 105 ---ADVMGEGMLRIAATRDWVSGES-SLPVSKRLSAXXXXXXXXXXXXXXXXXXXALKDE 160
              AD    G L++  T++W+  ES S P++K+  A                   ALK E
Sbjct: 112 IDGADDDESGFLKLKPTQEWIGWESDSAPMNKKALAKALRDDSERRKKFNFLKYEALKRE 171

Query: 161 LLLLTTGIGAACSLYCLLVFSLEAAVSYAFGVAFSCLYLQLLYRHTDNLSKKDVPEIFMK 220
           L+ L+  IG  CS YCLL  S++AAVSYA GV FSCLYLQLLY + D LS++ VP+IF+K
Sbjct: 172 LMYLSIVIGTGCSGYCLLALSVQAAVSYAVGVLFSCLYLQLLYGYADGLSREAVPDIFLK 231

Query: 221 KKVKRIGIRSEDLKNTIEKVLGGISVALSSPRLVIPAIFFGLSTLS-DHFQNSILNFELV 279
           KK K+IGIRSEDL++ + + + G  +ALSSPRLVIPA  +GL  LS  +FQN + +F+  
Sbjct: 232 KKSKKIGIRSEDLEDFVVRTIRGSGMALSSPRLVIPAAIYGLWILSHKYFQNDLFDFQFQ 291

Query: 280 PGMMGFFA 287
             ++  + 
Sbjct: 292 QWLVCLYT 299



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.137    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,690,463
Number of extensions: 203864
Number of successful extensions: 444
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 443
Number of HSP's successfully gapped: 1
Length of query: 315
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 217
Effective length of database: 8,419,801
Effective search space: 1827096817
Effective search space used: 1827096817
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 112 (47.8 bits)