BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0322000 Os03g0322000|AY224484
         (106 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G55060.1  | chr3:20403189-20406222 REVERSE LENGTH=897           51   1e-07

>AT3G55060.1 | chr3:20403189-20406222 REVERSE LENGTH=897
          Length = 896

 Score = 51.2 bits (121), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 33/106 (31%), Positives = 55/106 (51%)

Query: 1   EKSDVQSTLEMELDRRSNDWSVKLAEFQSXXXXXXXXXXXXXXXNVSFQREVTLLESNRI 60
           EKS++Q+ LE ELDRRS +W+ KL +FQ                NVS QRE++    N  
Sbjct: 396 EKSELQAGLEKELDRRSGEWTSKLEKFQLEEKKLRERVRELAEHNVSLQRELSAFHENET 455

Query: 61  DVSNKITSLELQNKQLNDELQKVKKEHDTLLKSSVELNDNLTKTAE 106
           +  + IT LE +  +L     K+ +E++ + ++  +L ++     E
Sbjct: 456 ENKDMITHLERRVAELTTTADKLHEENNYVKQTLSKLQESYAGATE 501



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.303    0.120    0.301 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,526,616
Number of extensions: 40026
Number of successful extensions: 311
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 309
Number of HSP's successfully gapped: 2
Length of query: 106
Length of database: 11,106,569
Length adjustment: 75
Effective length of query: 31
Effective length of database: 9,050,369
Effective search space: 280561439
Effective search space used: 280561439
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.9 bits)
S2: 105 (45.1 bits)