BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0320100 Os03g0320100|AK062471
         (273 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G10740.1  | chr3:3361031-3364573 REVERSE LENGTH=679            268   2e-72
AT5G26120.1  | chr5:9121835-9125399 REVERSE LENGTH=675            239   8e-64

>AT3G10740.1 | chr3:3361031-3364573 REVERSE LENGTH=679
          Length = 678

 Score =  268 bits (685), Expect = 2e-72,   Method: Compositional matrix adjust.
 Identities = 144/271 (53%), Positives = 187/271 (69%), Gaps = 4/271 (1%)

Query: 1   AIKASYPDXXXXXXXXXXXXXAVNPADLYDVHVYTSSGDMFSKTRMFDSTPRSGPKAFVS 60
           AIK +YPD               +PAD YD H+YTS+ ++FS    FD T R GPKAFVS
Sbjct: 405 AIKKAYPDIKIISNCDGSSHPLDHPADYYDYHIYTSASNLFSMYHQFDRTSRKGPKAFVS 464

Query: 61  EYAVTGNDAGRGTLVAALAEAAFLIGLERNSDVVEMASCAPLFINDNDRGFSPDAIVFNS 120
           EYAVTG DAG G+L+A+LAEAAFLIGLE+NSD+VEMAS APLF+N NDR ++PDAIVFNS
Sbjct: 465 EYAVTGKDAGTGSLLASLAEAAFLIGLEKNSDIVEMASYAPLFVNTNDRRWNPDAIVFNS 524

Query: 121 WQHYGCPNYWMLHFFKDSSGATLHPLTIQVSNYDQLAASALTWQNSNDGNTYLKIKVVNF 180
              YG P+YW+  FF +SSGATL   T++  N   L ASA++W+  N+G  Y++IK VNF
Sbjct: 525 SHLYGTPSYWVQRFFAESSGATLLTSTLK-GNSTSLVASAISWK--NNGKDYIRIKAVNF 581

Query: 181 GNKAVNLNIAVAGLE-NGIQEFGSIKTVLTSGWLRDENSFQQPDKVVPAASPITNAGEKM 239
           G  + N+ + V GL+ N ++  GS KTVLTS  + DENSF QP+KVVP  S +  A E M
Sbjct: 582 GANSENMQVLVTGLDPNVMRVSGSKKTVLTSTNVMDENSFSQPEKVVPHESLLELAEEDM 641

Query: 240 GVIVDPYSLTSFDLLLDTNTDKYPLLESSFH 270
            V++ P+S +SFDLL ++   + P+ +SS H
Sbjct: 642 TVVLPPHSFSSFDLLKESAKIRMPISDSSSH 672



>AT5G26120.1 | chr5:9121835-9125399 REVERSE LENGTH=675
          Length = 674

 Score =  239 bits (611), Expect = 8e-64,   Method: Compositional matrix adjust.
 Identities = 133/269 (49%), Positives = 178/269 (66%), Gaps = 7/269 (2%)

Query: 1   AIKASYPDXXXXXXXXXXXXXAVNPADLYDVHVYTSSGDMFSKTRMFDSTPRSGPKAFVS 60
           AIK +YPD               +PAD +D H+YT + D+FSK+  FD+TPR+GPKAFVS
Sbjct: 404 AIKKAYPDIKIISNCDASAKPLDHPADYFDYHIYTLARDLFSKSHDFDNTPRNGPKAFVS 463

Query: 61  EYAVTGNDAGRGTLVAALAEAAFLIGLERNSDVVEMASCAPLFINDNDRGFSPDAIVFNS 120
           EYAV   DA  G L+AAL EAAFL+GLE+NSD+VEM S APLF+N NDR + PDAIVFNS
Sbjct: 464 EYAVNKADAKNGNLLAALGEAAFLLGLEKNSDIVEMVSYAPLFVNTNDRRWIPDAIVFNS 523

Query: 121 WQHYGCPNYWMLHFFKDSSGATLHPLTIQVSNYDQLAASALTWQNSNDGNTYLKIKVVNF 180
              YG P+YW+ HFF +SSGATL   T++      + ASA+++Q   +G  Y++IK VNF
Sbjct: 524 SHLYGTPSYWVQHFFTESSGATLLNSTLK-GKTSSVEASAISFQ--TNGKDYIQIKAVNF 580

Query: 181 GNKAVNLNIAVAGLENGIQEFGSIKTVLTSGWLRDENSFQQPDKVVPAASPI-TNAGEKM 239
           G ++VNL +AV GL    + +GS K VLTS  + DENSF  P+ +VP  S +     E +
Sbjct: 581 GEQSVNLKVAVTGLM--AKFYGSKKKVLTSASVMDENSFSNPNMIVPQESLLEMTEQEDL 638

Query: 240 GVIVDPYSLTSFDLLLDT-NTDKYPLLES 267
             ++ P+S +SFDLL ++ N  K P+ +S
Sbjct: 639 MFVLPPHSFSSFDLLTESENVIKMPISDS 667



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.316    0.132    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,903,499
Number of extensions: 245468
Number of successful extensions: 595
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 589
Number of HSP's successfully gapped: 2
Length of query: 273
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 176
Effective length of database: 8,447,217
Effective search space: 1486710192
Effective search space used: 1486710192
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 111 (47.4 bits)