BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0313000 Os03g0313000|AK068580
         (173 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G52840.1  | chr5:21413718-21414794 FORWARD LENGTH=170          224   2e-59
AT4G28005.1  | chr4:13929655-13930191 REVERSE LENGTH=116           86   1e-17

>AT5G52840.1 | chr5:21413718-21414794 FORWARD LENGTH=170
          Length = 169

 Score =  224 bits (571), Expect = 2e-59,   Method: Compositional matrix adjust.
 Identities = 109/154 (70%), Positives = 130/154 (84%), Gaps = 4/154 (2%)

Query: 23  KETTGIVGLEVVPNAREVLVGLYTRTLKEIEAVPKDEGYRKAVESFTRHRLQICQEEDDW 82
           K+TTGIVGL+VVPNAR VL+ LY++TLKEI+AVP+DEGYRKAVESFTR RL +C+EE+DW
Sbjct: 15  KQTTGIVGLDVVPNARAVLIDLYSKTLKEIQAVPEDEGYRKAVESFTRQRLNVCKEEEDW 74

Query: 83  KRIEDRIGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVIEDDTLIPKHVPQHR 142
           + IE R+GCGQVEELIEEA+DEL LI KMIEWDPWGVPDDYECEVIE+D  IPKHVPQHR
Sbjct: 75  EMIEKRLGCGQVEELIEEARDELTLIGKMIEWDPWGVPDDYECEVIENDAPIPKHVPQHR 134

Query: 143 PVALPEEFFKTLDAV----KSDPALQGESPPQVK 172
           P  LPE+F+KTL+ +    K++      S PQ+K
Sbjct: 135 PGPLPEQFYKTLEGLIAESKTEIPAATPSDPQLK 168



>AT4G28005.1 | chr4:13929655-13930191 REVERSE LENGTH=116
          Length = 115

 Score = 85.5 bits (210), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 49/104 (47%), Positives = 62/104 (59%), Gaps = 14/104 (13%)

Query: 73  LQICQEEDDWKRIEDRIGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVIEDDT 132
           +++C EE+DW  IE R+ CG+VEELIEEA DEL L+ KMI              +I    
Sbjct: 21  VEVCPEEEDWGVIEKRLACGKVEELIEEASDELTLVGKMI--------GSLHPNLILAP- 71

Query: 133 LIPKHVPQHRPVALPEEFFKTLDAVKSDPALQ----GESPPQVK 172
            IPKHVP HRP  LPEEF++TL AV S+   +      S PQ+K
Sbjct: 72  -IPKHVPHHRPGPLPEEFYRTLQAVNSESKTEITATSSSDPQLK 114



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.317    0.138    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,771,354
Number of extensions: 161281
Number of successful extensions: 458
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 457
Number of HSP's successfully gapped: 2
Length of query: 173
Length of database: 11,106,569
Length adjustment: 92
Effective length of query: 81
Effective length of database: 8,584,297
Effective search space: 695328057
Effective search space used: 695328057
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 108 (46.2 bits)