BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0310800 Os03g0310800|AK099476
         (173 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G49480.1  | chr5:20070812-20071294 FORWARD LENGTH=161          101   2e-22

>AT5G49480.1 | chr5:20070812-20071294 FORWARD LENGTH=161
          Length = 160

 Score =  101 bits (251), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 59/165 (35%), Positives = 86/165 (52%), Gaps = 10/165 (6%)

Query: 1   MCPGGRYAGLDVPACAAAG-DLRPAFDVLDADRDGRISREDLKSFYASGAATSERFXXXX 59
           MCP GR A   +P    A  + RPAF+++D D DG+IS +DL++FYA G  + E      
Sbjct: 1   MCPSGRIA---IPITTTANPNFRPAFEIIDTDHDGKISSDDLRAFYA-GIPSGENNDETM 56

Query: 60  XXXXXXXXXXXXXGFVQYDEFEXXXXXXXXXXXCRPA----MEDVLRVMDRDGDGKVGFD 115
                        GFV++DEFE                   M+DV +VMD+DGDG++ + 
Sbjct: 57  IGTMISVADANKDGFVEFDEFEKVLETTPFSRSGNGGDDGLMKDVFKVMDKDGDGRLSYG 116

Query: 116 DLKAYLGWAGMPAADEEIRAMIRVAXXXXXXXCVGLEELAIVLGC 160
           DLK+Y+  AG+   D+EI++MIR+A        V  + L  + GC
Sbjct: 117 DLKSYMDSAGLAVTDDEIKSMIRLAGGDLNDG-VSFDGLLKIFGC 160



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.138    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,972,156
Number of extensions: 94424
Number of successful extensions: 480
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 479
Number of HSP's successfully gapped: 1
Length of query: 173
Length of database: 11,106,569
Length adjustment: 92
Effective length of query: 81
Effective length of database: 8,584,297
Effective search space: 695328057
Effective search space used: 695328057
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 108 (46.2 bits)