BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0305600 Os03g0305600|AK063714
         (179 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G16160.2  | chr4:9157545-9158738 FORWARD LENGTH=179            176   5e-45
AT2G28900.1  | chr2:12414423-12415459 REVERSE LENGTH=149           64   5e-11

>AT4G16160.2 | chr4:9157545-9158738 FORWARD LENGTH=179
          Length = 178

 Score =  176 bits (446), Expect = 5e-45,   Method: Compositional matrix adjust.
 Identities = 93/173 (53%), Positives = 118/173 (68%), Gaps = 6/173 (3%)

Query: 12  RTFLDEVRGLEKNWMVDLGHPLLNRVAESFVKAAGIGAVQAVARESYFXXXXXXXXXXXX 71
           R  +DE+R  EK  + DLGHPLLNR+A+SFVKAAG+GA+QAV+RE+YF            
Sbjct: 7   RIVMDEIRSFEKAHLFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGAGFDSNN 66

Query: 72  V---SDSTGARKRSFPDLNGGNSSKSAEAMVKSVSKESLQWGLAAGLHSGLTYGLTEAR- 127
           V   S+ TG +K  FP+L  G SSKS +A+VK+  KESLQWGLAAGL+SG+TYG+TE R 
Sbjct: 67  VGPPSEITGNKKHRFPNLR-GESSKSLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRG 125

Query: 128 GTHDWKNSXXX-XXXXXXXXXXXSDRASHERVVQCAIAGAALSTAANVLSGIL 179
           G HDW+NS               S+R SHE+VVQ A+ GAA+STAAN+LS + 
Sbjct: 126 GAHDWRNSAVAGALTGAAMAMTTSERTSHEQVVQSALTGAAISTAANLLSSVF 178



>AT2G28900.1 | chr2:12414423-12415459 REVERSE LENGTH=149
          Length = 148

 Score = 63.5 bits (153), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 43/169 (25%), Positives = 75/169 (44%), Gaps = 27/169 (15%)

Query: 9   LETRTFLDEVRGLEKNWMVDLGHPLLNRVAESFVKAAGIGAVQAVARESYFXXXXXXXXX 68
           + + TF   V   + +  VD+G+P LN   ++F+K   +G  +++A ++Y          
Sbjct: 1   MPSSTFSGTVSTPKLSVAVDMGNPFLNLTVDAFLKIGAVGVTKSLAEDTY---------- 50

Query: 69  XXXVSDSTGARKRSFPDLNGGNSSKSA-EAMVKSVSKESLQWGLAAGLHSGLTYGLTEAR 127
                            ++ G+ SKS  E  +K + KE + WG A G++ G  YG+   R
Sbjct: 51  ---------------KAIDKGSLSKSTLEHALKKLCKEGVYWGAAGGVYIGTEYGIERIR 95

Query: 128 GTHDWKNSXXXXXXXXXXXXXXSDRASHERVVQCAIAGAALSTAANVLS 176
           G+ DWKN+                +     V+  AI G AL+TA+  ++
Sbjct: 96  GSRDWKNAMLAGAATGAVLSAVGKKGKDTIVID-AILGGALATASQFVN 143



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.312    0.125    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,780,441
Number of extensions: 76806
Number of successful extensions: 175
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 171
Number of HSP's successfully gapped: 2
Length of query: 179
Length of database: 11,106,569
Length adjustment: 92
Effective length of query: 87
Effective length of database: 8,584,297
Effective search space: 746833839
Effective search space used: 746833839
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 108 (46.2 bits)