BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0302800 Os03g0302800|AK061293
         (214 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G38700.1  | chr5:15489690-15490238 FORWARD LENGTH=183           65   3e-11
AT4G02170.1  | chr4:958126-958641 FORWARD LENGTH=172               55   3e-08

>AT5G38700.1 | chr5:15489690-15490238 FORWARD LENGTH=183
          Length = 182

 Score = 65.1 bits (157), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 52/140 (37%), Positives = 64/140 (45%), Gaps = 35/140 (25%)

Query: 72  EREYSIEETPAFRFRTPSARVLR-LIPCIA-PAVPDTPGLYGDEDRYFFARRDTEPECGG 129
           E++ S +ETP F  +      +R L+PCIA PA  D          Y F    T+ +   
Sbjct: 69  EKQLSFDETPIFNVKMHRPTSMRFLLPCIARPADFD----------YDFELDGTQDDTDD 118

Query: 130 --GVGYYDYNGEPGECGGVDDESFRDGXXXXXXXXXXXXXXXXXXVTEDAGVDAKAEEFI 187
               GYYD     G C    D S  D                     E+ GVD +AEEFI
Sbjct: 119 VRSYGYYD-----GSCNEKCDRSANDDQEEE----------------EEKGVDVRAEEFI 157

Query: 188 AKFHAQMKLQRQISWLQYNE 207
           AKF+ QMKLQRQIS+LQY E
Sbjct: 158 AKFYEQMKLQRQISYLQYKE 177



>AT4G02170.1 | chr4:958126-958641 FORWARD LENGTH=172
          Length = 171

 Score = 54.7 bits (130), Expect = 3e-08,   Method: Compositional matrix adjust.
 Identities = 48/142 (33%), Positives = 63/142 (44%), Gaps = 50/142 (35%)

Query: 72  EREYSIEETPAFRFR------TPSARVLRLIPCIAPAVPDTPGLYGDEDRYFFARRDTEP 125
           ER+ S +ETP F  +      T S R L L+PC AP V                  D + 
Sbjct: 67  ERQLSFDETPLFNVKKKMHCPTSSMRFL-LLPCFAPPVD----------------FDYDF 109

Query: 126 ECGGGVGYYDYNGEPGECGGVDDESFRDGXXXXXXXXXXXXXXXXXXVTEDAGVDAKAEE 185
           E  G     DY+ E    G   DES  +                     E+ GVD +AEE
Sbjct: 110 EMDGQ----DYSDEVQSYGY--DESCSE---------------------EEKGVDVRAEE 142

Query: 186 FIAKFHAQMKLQRQISWLQYNE 207
           FIAKF+ Q+KLQRQ+S+L+Y +
Sbjct: 143 FIAKFYEQIKLQRQVSYLKYKQ 164



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.138    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,241,623
Number of extensions: 122592
Number of successful extensions: 205
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 205
Number of HSP's successfully gapped: 2
Length of query: 214
Length of database: 11,106,569
Length adjustment: 94
Effective length of query: 120
Effective length of database: 8,529,465
Effective search space: 1023535800
Effective search space used: 1023535800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 109 (46.6 bits)