BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0297400 Os03g0297400|AK101597
         (387 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G30200.1  | chr2:12883162-12885482 REVERSE LENGTH=394          538   e-153

>AT2G30200.1 | chr2:12883162-12885482 REVERSE LENGTH=394
          Length = 393

 Score =  538 bits (1386), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 261/333 (78%), Positives = 289/333 (86%), Gaps = 1/333 (0%)

Query: 55  RSRVSAAGVSLGSEVAVGSDALFADYKPTTAFLFPGQGAQTVGMGAEAVNVPAAAKLFDK 114
           RSR+S + VS GS+     D+LFADYKPT+AFLFPGQGAQ VGMG E+ +V AA +L+ K
Sbjct: 62  RSRISMS-VSAGSQSTTVHDSLFADYKPTSAFLFPGQGAQAVGMGKESQSVGAAGELYKK 120

Query: 115 ANDILGYDLLDFCTNGPKEKLDSTVISQPAIYVTSLAAVEVLRSRDGGQNVIDSVDVTCG 174
           ANDILGYDLLD C NGPKEKLDSTVISQPAIYVTSLAAVE+LR R+GG+ +I+SVDVTCG
Sbjct: 121 ANDILGYDLLDICVNGPKEKLDSTVISQPAIYVTSLAAVELLRVREGGEQIINSVDVTCG 180

Query: 175 LSLGEYTALAFAGAFSFEDGLKLVKLRGEXXXXXXXXXXXXXXXXIGLDSEKVQQLCDAA 234
           LSLGEYTALAFAGAFSFEDGLKLVKLRGE                IGLDSEKVQQLCDAA
Sbjct: 181 LSLGEYTALAFAGAFSFEDGLKLVKLRGEAMQAAADAAKSAMVSIIGLDSEKVQQLCDAA 240

Query: 235 NEEVDEKERVQIANFLCPGNYAVSGGVKGIEAVEAKAKSFKARMTVRLAVAGAFHTSFMQ 294
           N+EVDE ++VQIAN+LCPGNYAVSGG+KGIE VEAKAKSFKARMTVRLAVAGAFHTSFM+
Sbjct: 241 NQEVDEADKVQIANYLCPGNYAVSGGLKGIEVVEAKAKSFKARMTVRLAVAGAFHTSFME 300

Query: 295 PAVSRLESALAETEIKTPRIPVISNVDASPHSDPDTIKKILARQVTSPVQWESTVKTLMG 354
           PAVSRLE+ALA TEI++PRIPVISNVDA PH+DPDTIKKILARQVTSPVQWE+TVKTL+ 
Sbjct: 301 PAVSRLEAALAATEIRSPRIPVISNVDAQPHADPDTIKKILARQVTSPVQWETTVKTLLS 360

Query: 355 KGLEKSYELGPGKVIAGILKRIDKGASIENIGA 387
           KGL+ SYELGPGKVIAGI KR+DK AS ENI A
Sbjct: 361 KGLKSSYELGPGKVIAGIFKRVDKSASFENISA 393



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.131    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,440,242
Number of extensions: 242614
Number of successful extensions: 436
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 435
Number of HSP's successfully gapped: 1
Length of query: 387
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 287
Effective length of database: 8,364,969
Effective search space: 2400746103
Effective search space used: 2400746103
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 113 (48.1 bits)