BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0293900 Os03g0293900|AK072637
(49 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT2G28000.1 | chr2:11926603-11929184 FORWARD LENGTH=587 54 1e-08
AT5G18820.1 | chr5:6271549-6274153 FORWARD LENGTH=576 47 2e-06
>AT2G28000.1 | chr2:11926603-11929184 FORWARD LENGTH=587
Length = 586
Score = 54.3 bits (129), Expect = 1e-08, Method: Composition-based stats.
Identities = 29/49 (59%), Positives = 35/49 (71%)
Query: 1 IGGEVVVQKIEDSECKVSYNAMNIKYENSIKASVINPAKVRRCMLQNAA 49
+ GEVVV+KI S+ + YNAM YEN +A VI+PAKV RC LQNAA
Sbjct: 506 VEGEVVVEKIMFSDWENGYNAMTDTYENLFEAGVIDPAKVTRCALQNAA 554
>AT5G18820.1 | chr5:6271549-6274153 FORWARD LENGTH=576
Length = 575
Score = 47.4 bits (111), Expect = 2e-06, Method: Compositional matrix adjust.
Identities = 22/48 (45%), Positives = 31/48 (64%)
Query: 1 IGGEVVVQKIEDSECKVSYNAMNIKYENSIKASVINPAKVRRCMLQNA 48
+ G VVVQK + E + YNAM+ KYE+ + A + +P +V R LQNA
Sbjct: 494 VDGSVVVQKTRELEWRSGYNAMSGKYEDLLNAGIADPCRVSRFALQNA 541
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.314 0.127 0.347
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 931,225
Number of extensions: 21591
Number of successful extensions: 68
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 68
Number of HSP's successfully gapped: 2
Length of query: 49
Length of database: 11,106,569
Length adjustment: 23
Effective length of query: 26
Effective length of database: 10,476,001
Effective search space: 272376026
Effective search space used: 272376026
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 104 (44.7 bits)