BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0284400 Os03g0284400|AK069064
         (220 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G13510.1  | chr5:4341294-4341956 FORWARD LENGTH=221            277   2e-75
AT3G12370.1  | chr3:3937464-3937979 REVERSE LENGTH=172            186   7e-48

>AT5G13510.1 | chr5:4341294-4341956 FORWARD LENGTH=221
          Length = 220

 Score =  277 bits (709), Expect = 2e-75,   Method: Compositional matrix adjust.
 Identities = 127/183 (69%), Positives = 155/183 (84%), Gaps = 1/183 (0%)

Query: 37  ISAAITRGRKEDTVAAVREQLEGCYLLAGIRYEGLTVKQLQGIRDTLPETCRLLVAKNTL 96
           I +A++R +KE+TV AV+  LE C+LLA I Y+GLTVKQ Q +R TLP+T +L+VAKNTL
Sbjct: 39  IRSAVSRNKKEETVEAVKSHLENCHLLAAINYKGLTVKQFQDLRRTLPDTTKLIVAKNTL 98

Query: 97  VGKAIEGTPWEALKPCMKGMNAWLFVHTEEVPAALKPYRAFQKEERLEETNDFVGAVFEG 156
           V KAIEGT WEALKPCMKGMNAWLFV T+E+P+A+KPYR+FQKE +L E NDF GAVFEG
Sbjct: 99  VFKAIEGTKWEALKPCMKGMNAWLFVQTDEIPSAIKPYRSFQKERKL-ENNDFAGAVFEG 157

Query: 157 KFYAPGDFKALETMPSRAEVYAKLLGSLQGPATSVVTTLQAPARDVVSVLSAYVRKLEQE 216
           KFYAP +FK LETMP+RAEVYAK+LG+LQ PA ++VTTLQAPAR+V+ VL AY++KLE E
Sbjct: 158 KFYAPDNFKVLETMPTRAEVYAKMLGALQSPAINLVTTLQAPAREVIMVLMAYIKKLEDE 217

Query: 217 VGA 219
             A
Sbjct: 218 SNA 220



>AT3G12370.1 | chr3:3937464-3937979 REVERSE LENGTH=172
          Length = 171

 Score =  186 bits (473), Expect = 7e-48,   Method: Compositional matrix adjust.
 Identities = 89/145 (61%), Positives = 109/145 (75%), Gaps = 3/145 (2%)

Query: 56  QLEGCYLLAGIRYEGLTVKQLQGIRDTLPETC--RLLVAKNTLVGKAIEGTPWEALKPCM 113
            LE C+ + GI Y GL+VKQLQ +R  L E    +LLVAKNTLV KA+EGT WE+LKPCM
Sbjct: 17  NLENCHFITGINYNGLSVKQLQELRGILRENTNTKLLVAKNTLVYKALEGTKWESLKPCM 76

Query: 114 KGMNAWLFVHTEEVPAALKPYRAFQKEERLEETNDFVGAVFEGKFYAPGDFKALETMPSR 173
           KGMNAWLFV +E++PAALK +  FQKE++L + N+  GAVFE K YAP D+K +ETMPSR
Sbjct: 77  KGMNAWLFVQSEDIPAALKTFINFQKEKKLYD-NNLGGAVFEEKLYAPQDYKVIETMPSR 135

Query: 174 AEVYAKLLGSLQGPATSVVTTLQAP 198
           A+VY  +LGSL  PA  +V  LQAP
Sbjct: 136 ADVYGMMLGSLHWPALDLVNALQAP 160



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.317    0.132    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,069,187
Number of extensions: 155530
Number of successful extensions: 302
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 299
Number of HSP's successfully gapped: 2
Length of query: 220
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 125
Effective length of database: 8,502,049
Effective search space: 1062756125
Effective search space used: 1062756125
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 109 (46.6 bits)