BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0282800 Os03g0282800|AK101658
         (224 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G53490.1  | chr3:19832666-19834024 FORWARD LENGTH=226          182   1e-46
AT5G02720.1  | chr5:613782-614313 REVERSE LENGTH=139               67   6e-12

>AT3G53490.1 | chr3:19832666-19834024 FORWARD LENGTH=226
          Length = 225

 Score =  182 bits (463), Expect = 1e-46,   Method: Compositional matrix adjust.
 Identities = 96/194 (49%), Positives = 118/194 (60%), Gaps = 2/194 (1%)

Query: 17  VVLFVALCSIPGIFSQRLVTLDSIDIFTTHEWFPSKPTVYFLCNGEDKVYLPDVKDANNI 76
           ++ F+   S+PG    + VTLDS+ IFTTH+WF +KPTV+F C GE+K  LPDVK  N  
Sbjct: 16  LLCFIFFSSLPGTILTQEVTLDSVQIFTTHDWFSTKPTVFFQCKGENKTVLPDVKRTNVS 75

Query: 77  YTFKGEESWQPLTELPEKKCKRCGLYEEDTFKHDLYDEWELCSSDF-KKGKYTHFKEGQF 135
           Y+F GEESWQPLTEL   KCKRCG+YE+D  K+D +DEWELC SDF  +G Y  FKE +F
Sbjct: 76  YSFNGEESWQPLTELQGTKCKRCGIYEDDPLKYDTFDEWELCPSDFTAEGSYKRFKEKEF 135

Query: 136 NATFLCPNCT-ASAGDSANHDXXXXXXXXXXXXXXXXXXXXXXXXXXXXALFGGYKYWLK 194
           NATFLC  C+   AG +                                 L  GYKYW K
Sbjct: 136 NATFLCHGCSQVGAGSNKESGTEKEEQKGGMHPGIVVLIVVLLLGVVAVGLLVGYKYWRK 195

Query: 195 KKRERDQLRFLKLF 208
           KKR+++Q RFLKLF
Sbjct: 196 KKRQQEQARFLKLF 209



>AT5G02720.1 | chr5:613782-614313 REVERSE LENGTH=139
          Length = 138

 Score = 67.4 bits (163), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 38/123 (30%), Positives = 58/123 (47%), Gaps = 3/123 (2%)

Query: 88  LTELPEKKCKRCGLYEEDTFKHDL-YDEWELCSSDFKKGK-YTHFKEGQFNATFLCPNCT 145
           +T +  +KCKRCG+YE+ +   D  +D WE+C +DF   + Y HFKE + NATF+C  C 
Sbjct: 1   MTGIGGEKCKRCGIYEQGSLVSDKEFDVWEVCPTDFSASQVYMHFKEKEINATFVCHGCA 60

Query: 146 ASAGDSANHDXXXXXXXXXXXXXXXXXXXXXXXXXXXXALFGGYKYWLKKKRERDQLRFL 205
                 A                                +F  +K+  + K++RDQ RF+
Sbjct: 61  KFHSAVAASSPQEEGYNGLTFMIAIIAGVLCTTLVVVGGVF-MFKHTQRMKKQRDQARFM 119

Query: 206 KLF 208
           +LF
Sbjct: 120 QLF 122



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.141    0.466 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,690,436
Number of extensions: 194781
Number of successful extensions: 402
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 398
Number of HSP's successfully gapped: 2
Length of query: 224
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 129
Effective length of database: 8,502,049
Effective search space: 1096764321
Effective search space used: 1096764321
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 110 (47.0 bits)