BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0262900 Os03g0262900|AK070551
         (371 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G19770.1  | chr3:6866916-6869114 FORWARD LENGTH=521            351   4e-97
AT5G09320.1  | chr5:2889007-2892016 REVERSE LENGTH=713            332   3e-91

>AT3G19770.1 | chr3:6866916-6869114 FORWARD LENGTH=521
          Length = 520

 Score =  351 bits (900), Expect = 4e-97,   Method: Compositional matrix adjust.
 Identities = 190/356 (53%), Positives = 249/356 (69%), Gaps = 27/356 (7%)

Query: 1   MTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIKPEYQSETSWLLAQKELQKI 60
           MTKLF RVFAS  E+V +DE+LF+KMSL+QQFI PENLDI+P +Q+E+SWLLAQKELQKI
Sbjct: 87  MTKLFTRVFASNTEEVIADEKLFQKMSLVQQFISPENLDIQPTFQNESSWLLAQKELQKI 146

Query: 61  NMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEFLPVLIYVTIK--------- 111
           NMYKAPRDKL CILNCCKVINNLLLNASI SNEN PGADEFLPVLIYVTIK         
Sbjct: 147 NMYKAPRDKLVCILNCCKVINNLLLNASIASNENAPGADEFLPVLIYVTIKANPPQLHSN 206

Query: 112 -----RYRRQSRLVSEAQYFFTNILSAESFIWNIDGESLSMDERDFQKKMDLARERMLGL 166
                RYRR+S+LV EA YFFTNILSAESFI NID +S+S+DE +F+K M+ AR R+ GL
Sbjct: 207 LLYIQRYRRESKLVGEAAYFFTNILSAESFISNIDAKSISLDEAEFEKNMESARARISGL 266

Query: 167 SASSENQDNQNNLDVRE----QKSQTLKASRDSDVNLS-LKDNFQGPGLEMRRDSDASSN 221
            + +    + +    R+    QK+Q+L   R++ +  S   D+  G    +  +S+    
Sbjct: 267 DSQTYQTGHGSAPPPRDESTLQKTQSLNPKRENTLFQSKSSDSLSGTNELLNINSET--- 323

Query: 222 PVERVQSISDLEKKGAAELLKDDDLNKKIQEYPFLFARSGDLTVADVENLLNSYKQLVLK 281
           P+++ +SISDLE KGA  LLKD + +K  QEYP++FA +GDL + DVE LLNSYKQLV K
Sbjct: 324 PMKKAESISDLENKGAT-LLKDTEPSKVFQEYPYIFASAGDLRIGDVEGLLNSYKQLVFK 382

Query: 282 YVALSQGMG----INLENPPVQSMQTVSDLVESEEPKNVKNAVNFSEGSSKTSDDI 333
           YV L++G+G    +   + P+Q+    +   ESE+ +   + V  ++ + ++ DD+
Sbjct: 383 YVCLTKGLGDGTSLAPSSSPLQASSGFNTSKESEDHRRSSSDVQMTKETDRSVDDL 438



>AT5G09320.1 | chr5:2889007-2892016 REVERSE LENGTH=713
          Length = 712

 Score =  332 bits (850), Expect = 3e-91,   Method: Compositional matrix adjust.
 Identities = 178/307 (57%), Positives = 212/307 (69%), Gaps = 30/307 (9%)

Query: 1   MTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIKPEYQSETSWLLAQKELQKI 60
           MTKLF RVFAS  EDV SDE+LF+K+SL+QQFI PENLDI+P +Q++TSWLLAQKELQKI
Sbjct: 84  MTKLFPRVFASNTEDVISDEKLFQKISLVQQFISPENLDIQPTFQNQTSWLLAQKELQKI 143

Query: 61  NMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEFLPVLIYVTIK--------- 111
           NMY APRDKL CIL CCKVINNLLLNASI SN+N PGAD+FLPVLIYVTIK         
Sbjct: 144 NMYNAPRDKLMCILRCCKVINNLLLNASIASNQNEPGADQFLPVLIYVTIKANPPQFHSN 203

Query: 112 -----RYRRQSRLVSEAQYFFTNILSAESFIWNIDGESLSMDERDFQKKMDLARERMLGL 166
                RYRRQS+LV EA Y FTNILSAESFI NID +SLSMDE DF+ KM  A  R+ G 
Sbjct: 204 LLYIQRYRRQSKLVGEAGYLFTNILSAESFISNIDAKSLSMDEADFETKMKSAHARLSGP 263

Query: 167 SASSENQDNQNNLDVREQKSQTLKASRDSDVNLSLK--DNFQGPGLEMRRDSDASSNPVE 224
            + S   D+   L        T   ++  ++ L  K  D+F G       +   S  P++
Sbjct: 264 GSQSYQTDHGAAL-------PTAHNTKRENMLLHTKSTDSFSGT------NETLSETPIK 310

Query: 225 RVQSISDLEKKGAAELLKD-DDLNKKIQEYPFLFARSGDLTVADVENLLNSYKQLVLKYV 283
           +   I+DLE KGAA LL D  +  K  QEYP++FA  GDL + DVE+LLN+YKQLV KYV
Sbjct: 311 KADPITDLENKGAATLLNDRSEATKIFQEYPYMFASVGDLKIGDVEDLLNNYKQLVFKYV 370

Query: 284 ALSQGMG 290
            LS+G+G
Sbjct: 371 CLSKGLG 377



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.311    0.128    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,812,094
Number of extensions: 323579
Number of successful extensions: 1083
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 1076
Number of HSP's successfully gapped: 2
Length of query: 371
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 271
Effective length of database: 8,364,969
Effective search space: 2266906599
Effective search space used: 2266906599
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 112 (47.8 bits)