BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0259700 Os03g0259700|015-018-F05
         (464 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G73930.1  | chr1:27795347-27797534 REVERSE LENGTH=624          532   e-151

>AT1G73930.1 | chr1:27795347-27797534 REVERSE LENGTH=624
          Length = 623

 Score =  532 bits (1371), Expect = e-151,   Method: Compositional matrix adjust.
 Identities = 268/462 (58%), Positives = 331/462 (71%), Gaps = 16/462 (3%)

Query: 1   VAAWPAPVPGRPMELPIGSAALRVHX-----------XXXXXXXXXXXXXXXXXXSVPYG 49
           V+ WPAPVPG+ MELPIG+A L+V+                              SVP G
Sbjct: 164 VSMWPAPVPGKLMELPIGNAMLKVNLPPAHSLPLENGILYEESASSMAPLLPTNQSVPQG 223

Query: 50  LFHDADLFAAFRGXXXXXXXXXXXMVVGEPVLVVAPSPPQCSEAVAGLVSLVAPLLYSVD 109
           LFHDADLF  +RG           +++GEP+L++AP+PPQCSEAVA LVSLVAPL  SVD
Sbjct: 224 LFHDADLFGIYRGLLLQLWTLWELLLIGEPILIIAPTPPQCSEAVACLVSLVAPLFCSVD 283

Query: 110 FRPYFTIHDPDFARLNALAEGEVFPPMVLGVTNLFFLKSLKSIPNVVSVGSPNPNSTRVL 169
           FRPYFTIHDP FARLN+L EG+ FPPMVLGVTN+FFLK+L++IP+VVSVG+P PNS RV 
Sbjct: 284 FRPYFTIHDPGFARLNSLREGDTFPPMVLGVTNIFFLKALRNIPHVVSVGTPAPNSNRV- 342

Query: 170 PVGGQSHGSGNGINGTPGKLKLDKLAINKFSPTGLLNSIKLRREGPLCLMTEHKEALWST 229
                +  S   ++  P  L + +L++ +FSPT LLN++KLRR+GPLCLMTEHKEA+W+T
Sbjct: 343 ---AFTSRSAGKLSVKPEGLGVQQLSLRRFSPTNLLNAVKLRRDGPLCLMTEHKEAVWTT 399

Query: 230 YVSTTKPDTSVLNRLIDAGVSPRIEESMSVVNNEILRRHFLELTTNFLAPFGPYLRTTTP 289
           Y + TKPDTS+LNRLIDAG+SPR+EESMSVVNNEILRRHFLELTTNFLAPFGPY +   P
Sbjct: 400 YSAVTKPDTSILNRLIDAGMSPRVEESMSVVNNEILRRHFLELTTNFLAPFGPYFQVNAP 459

Query: 290 SEGSSPFVDPPLLPPFHADEFVNGLAARGAGKFLSKRMKSNWLDLYRRFLEGPNFMPWFX 349
           S+GSSP+V PP LP F ADEF++ L+ RG GKFLSKRMKSNW DLYRRFL GPNF PWF 
Sbjct: 460 SDGSSPYVTPPALPSFGADEFLSNLSGRGVGKFLSKRMKSNWPDLYRRFLRGPNFSPWFQ 519

Query: 350 XXXXXXXXXXXXXXXXXXMNVDIEKLMSKLSELERIDSFNAIERYLLREMENSRT-GATE 408
                             M  D+ ++ S+++ELE +DSFNAIE+++  E+++  + G  +
Sbjct: 520 RRRAVAEQEQRRLWRLARMKTDMRQITSQMNELEAVDSFNAIEKHISEEVKSLESGGGAD 579

Query: 409 SVTVCEKLKGDLRAAFSVLPKDMQQLLLSNPKRALLLQGSNE 450
           S    +KLK DL+A FSVLPKDMQQLLL NP+RA LLQ  +E
Sbjct: 580 SEGTFQKLKKDLQAVFSVLPKDMQQLLLLNPQRASLLQDPSE 621



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.137    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 9,349,290
Number of extensions: 386830
Number of successful extensions: 921
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 918
Number of HSP's successfully gapped: 1
Length of query: 464
Length of database: 11,106,569
Length adjustment: 102
Effective length of query: 362
Effective length of database: 8,310,137
Effective search space: 3008269594
Effective search space used: 3008269594
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 113 (48.1 bits)