BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0254200 Os03g0254200|Os03g0254200
         (544 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G19500.1  | chr5:6579019-6581699 FORWARD LENGTH=506            138   1e-32
AT2G33260.1  | chr2:14100840-14102252 REVERSE LENGTH=437          100   2e-21

>AT5G19500.1 | chr5:6579019-6581699 FORWARD LENGTH=506
          Length = 505

 Score =  138 bits (347), Expect = 1e-32,   Method: Compositional matrix adjust.
 Identities = 116/377 (30%), Positives = 179/377 (47%), Gaps = 51/377 (13%)

Query: 112 TSIGSGILAVPQRTAPAGFVPSAACMVTCWAFLVIEALLLVEINVHLRRXXXXXXXXXXX 171
           T++G+GILA+P  T  +GF+ SA   + CWAF+V+  LL+ E+NV+              
Sbjct: 120 TTVGAGILAIPAVTQESGFLASAVACILCWAFMVVTGLLVAEVNVNTMSELGSGG----- 174

Query: 172 XXXXXQLEVISLKSMAQETLGEWGGNLAAAAYLFLSYTSMVAYTSKSGEVLSSVVAGVPE 231
                    +SL SMA+ TLG  G  + + +YL + YT +VAY ++S  +L++ + G+P 
Sbjct: 175 ---------VSLVSMAKRTLGSVGVQVVSWSYLLIHYTLLVAYIARSSGILTNFL-GIPI 224

Query: 232 PVSGCXXXXXXXXXXXXXXXXXXDKVNQLLTFVMIGLLLTIEVSAVAFGAGLTLPA--NT 289
             S                       N +L F +I     +   AVA G  L   A    
Sbjct: 225 WESATLFSLIFGGLCFFGSQRFIGAANGVLVFGVIASFAAL--VAVASG-DLHWEALLKA 281

Query: 290 NWEQVPATLPVIIFTLVFHDIAPVICAYLGGDLVRIRLSILVGSIVPLLSLLVWDDIALS 349
           N+E VP ++P+I  + V+ ++ PV+C  L GDL R+R +I++G+ +PL   LVWD + L 
Sbjct: 282 NFEAVPMSVPIIALSFVYQNVVPVLCTDLEGDLPRVRTAIVLGTAIPLGLFLVWDAVILG 341

Query: 350 ---ISTDLDGFDILDMLNTEWSYTV-----VETFSLLAVGTSLIGTLLGASQFFIEQMTN 401
              + T +    ++D L    S +V     VE FSL A+ TS IG +LG S FF   +  
Sbjct: 342 SFPVDTGVAVEKMVDPLQQLRSSSVTVGPFVEAFSLFAIATSYIGFVLGLSDFF-SDLLK 400

Query: 402 LVSSSAQGHEEEALRHRGAKTAVDNNRLSYIAAGAVVAPTVLIAATVPNSFSIATDIAGG 461
           L S                     N  L Y+          +++   P  F  A D AG 
Sbjct: 401 LPSG-------------------QNKPLLYLLTLVPPL---VLSLLDPEIFFKALDFAGT 438

Query: 462 YCMTILYGVLPPLMAWA 478
           Y + +L+G+LP  M+W+
Sbjct: 439 YGVLVLFGILPAAMSWS 455



>AT2G33260.1 | chr2:14100840-14102252 REVERSE LENGTH=437
          Length = 436

 Score =  100 bits (249), Expect = 2e-21,   Method: Compositional matrix adjust.
 Identities = 110/405 (27%), Positives = 174/405 (42%), Gaps = 57/405 (14%)

Query: 112 TSIGSGILAVPQRTAPAGFVPSAACMVTCWAFLVIEALLLVEINVHLRRXXXXXXXXXXX 171
           T++G G+L +P  T  +G +PS   ++  W +++   LL+ E++                
Sbjct: 26  TAVGPGMLGLPAATIRSGSIPSTIALLCSWVYVISSILLVAELSFAAMEEDNAAE----- 80

Query: 172 XXXXXQLEVISLKSMAQETLGEWGGNLAAAAYLFLSYTSMVAYTSKSGEVLSSVVAGVPE 231
                    +S   +A ++ G   G   A  Y  LS++ MVA  S  G ++S     +  
Sbjct: 81  ---------VSFTGLATKSFGNKFGVFVAFVYASLSFSLMVACVSGIGSIVSQWFPSMNP 131

Query: 232 PVSGCXXXXXXXXXXXXXXXXXXDKVNQLLTFVM---IGLLLTIEVSAVAFGAGLTLPAN 288
            ++                    D  N+ L F+M   I  L+ I +S VA    L     
Sbjct: 132 FLANAIFPLVSGILIGFFPFNAIDFTNRGLCFLMLFSITSLVAIGLS-VARSNVLASFGQ 190

Query: 289 TNWE--QVPATLPVIIFTLVFHDIAPVICAYLGGDLVRIRLSILVGSIVPLLSLLVWDDI 346
           + W+   V   +PV++ TL FH I P IC   G  +   R +ILVG +VPL  +L W+ I
Sbjct: 191 SCWKVSMVLPAVPVMVLTLGFHVITPFICNLAGDSVSDARRAILVGGVVPLAMVLSWNLI 250

Query: 347 ALSI---------STDLDGFDILDMLNTEWSYTVVETFSLLAVGTSLIGTLLGASQFFIE 397
            L +         S+ +D   +L  +N   + + V+ F+  A+ TSLIG  +     F +
Sbjct: 251 VLGLARITVPAAPSSTIDPISLLLSVNPS-ALSAVQGFAFSALATSLIGYAVS----FPK 305

Query: 398 QMTN---LVSSSAQGHEE-----EALRHRGAKTAVDNNRLSY------------IAAGAV 437
           Q+ +   LVS  + G+        + + R  +T   N R SY            +    V
Sbjct: 306 QLLDTWKLVSKQSNGNGRLGSVSFSSKERDRRT---NGRASYNEPARARDGFEAVVMLFV 362

Query: 438 VAPTVLIAATVPNSFSIATDIAGGYCMTILYGVLPPLMAWAIASR 482
           +    LIA   P++FS A D AG Y    L+GVLPP MA+   SR
Sbjct: 363 LGVPALIATFFPSTFSRALDFAGVYANCFLFGVLPPAMAYIQQSR 407



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.134    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 9,120,871
Number of extensions: 320983
Number of successful extensions: 725
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 718
Number of HSP's successfully gapped: 2
Length of query: 544
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 441
Effective length of database: 8,282,721
Effective search space: 3652679961
Effective search space used: 3652679961
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 114 (48.5 bits)