BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0249800 Os03g0249800|AK120462
         (130 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G21180.1  | chr2:9074956-9075351 REVERSE LENGTH=132             62   9e-11

>AT2G21180.1 | chr2:9074956-9075351 REVERSE LENGTH=132
          Length = 131

 Score = 62.0 bits (149), Expect = 9e-11,   Method: Compositional matrix adjust.
 Identities = 42/131 (32%), Positives = 63/131 (48%), Gaps = 3/131 (2%)

Query: 1   MAYQRGETSSVVEAFTLSPLPYPXXXXXXXXXXXXGVSWFFTYEDFMEEAAEQLSWXXXX 60
           MAY+    SS++++F+LSPLPYP              SW+F+ E+  E A EQ+++    
Sbjct: 1   MAYEE-RRSSILDSFSLSPLPYPVLLILAVASVFLLSSWYFSLEEAAESAEEQINFALLL 59

Query: 61  XXXXXXXXIRWISSVDTFDGYFSFYPTERRWNRYDPGPA--EGSSPWGVAMXXXXXXXXA 118
                   +RW+SS++  D     + + RR     PG     GSSPWGVA          
Sbjct: 60  IPLFLIVLVRWLSSMENPDALLGMFSSSRRTTYVSPGAGGDGGSSPWGVAALIVLLLVLL 119

Query: 119 SFHSTFQDMWK 129
            + S+F +MW 
Sbjct: 120 QYQSSFLEMWS 130



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.320    0.133    0.457 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,395,037
Number of extensions: 73532
Number of successful extensions: 77
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 1
Length of query: 130
Length of database: 11,106,569
Length adjustment: 87
Effective length of query: 43
Effective length of database: 8,721,377
Effective search space: 375019211
Effective search space used: 375019211
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 106 (45.4 bits)