BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0249100 Os03g0249100|AK106841
         (445 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G12330.1  | chr1:4194673-4196627 FORWARD LENGTH=506            166   2e-41
AT5G12900.1  | chr5:4072151-4074445 REVERSE LENGTH=563            139   4e-33

>AT1G12330.1 | chr1:4194673-4196627 FORWARD LENGTH=506
          Length = 505

 Score =  166 bits (421), Expect = 2e-41,   Method: Compositional matrix adjust.
 Identities = 128/411 (31%), Positives = 172/411 (41%), Gaps = 83/411 (20%)

Query: 33  DEKYAHVATPLHNHRGGSGGAKKTPRRAKSEGGGGADPAAYVAAVSCSDCRFKQR----- 87
           +  + H  TPLH H G     K+     + +GG          AVSC+ CR         
Sbjct: 66  NNNHQHTLTPLH-HNGKPQTRKRHDDNDEDDGG----------AVSCNKCRPHHSHRDKF 114

Query: 88  ----LHAPASPG----PGAVIRSLFVSLXXXXXXXXXXXXX------XASGGDGGESEQW 133
               L +  +P     P  +I+S+F SL                    +S  D    EQW
Sbjct: 115 SVVPLESHNNPSFISSPNLIIKSIFQSLTRRSPKPSSATAALPPRSSSSSAADASREEQW 174

Query: 134 XXXXXXXXXXXXXXXXXXXXXXXXXXXXKHSLTELEMKLARLEARVLPTPT--------A 185
                                       K S+ ELE KL +LE       +         
Sbjct: 175 RLAVAELSHKLIQATKKKEDAVIEASRLKSSMAELEKKLNKLEIYCHNLKSGLDECSNKK 234

Query: 186 AAFPV----------ESFLXXXXXXXXXXXXLTRALSNHLRS--------------PVNP 221
            + P+          + FL            L+R+L++ LR+              P + 
Sbjct: 235 QSVPIRKDGFNDRIIQQFLVSVSESRSSIRALSRSLASQLRTVGGKVYERLSLLLQPFDV 294

Query: 222 GPN------------LESFLNRAFHADFE---LDTEGDVHTADPAGRCEANLAAYHSIAA 266
             N            LE+ L+RAF  DFE       G     +P  RCE+N A+++ +  
Sbjct: 295 KINSFAKNPKSLIFYLEAILSRAFFEDFEAPGFQKNGSTRILNPIDRCESNYASFNVLME 354

Query: 267 LTWEEVLLHGTKHYSEGLSRFCDAKMSEVVSSLGWARARAWPEPLLQAFFLAAKGVWGVR 326
           LTW+EVL  GTKH+SE  SRFCD KMS+VVS L W   RAWPEPLLQAFF A+K VW V 
Sbjct: 355 LTWDEVLSRGTKHFSEEFSRFCDRKMSDVVSMLSW--NRAWPEPLLQAFFGASKSVWLVH 412

Query: 327 LLARSVHPPLPVVRVDRGARFDSRFMEDAAAGRAGKLEPASVKMMVAPGFH 377
           LLA SV+P L + RV++  RFD  +ME+    R   L    V+ MV PGF+
Sbjct: 413 LLANSVNPGLQIFRVEKDDRFDPIYMEETGGERFKSL----VRAMVQPGFY 459



>AT5G12900.1 | chr5:4072151-4074445 REVERSE LENGTH=563
          Length = 562

 Score =  139 bits (349), Expect = 4e-33,   Method: Compositional matrix adjust.
 Identities = 70/159 (44%), Positives = 98/159 (61%), Gaps = 8/159 (5%)

Query: 225 LESFLNRAFHADFE---LDTEGDVHTADPAGRCEANLAAYHSIAALTWEEVLLHGTKHYS 281
           LE+ ++++ + DFE       G     DP    +AN +++ S+  L+W EVL  GTK+YS
Sbjct: 388 LEAIISQSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYS 447

Query: 282 EGLSRFCDAKMSEVVSSLGWARARAWPEPLLQAFFLAAKGVWGVRLLARSVHPPLPVVRV 341
           +  SRFCD KMS ++++L W   R W E +LQAFF+AAK VW + LLA S +P L ++RV
Sbjct: 448 DEFSRFCDEKMSLIITTLNW--TRPWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRV 505

Query: 342 DRGARFDSRFMEDAAAGR---AGKLEPASVKMMVAPGFH 377
           +    F+S FMED  A R   A    PA VK+MV PGF+
Sbjct: 506 EENREFESSFMEDMGADRQRSALSRGPARVKVMVMPGFY 544



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.317    0.132    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,098,133
Number of extensions: 306281
Number of successful extensions: 776
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 773
Number of HSP's successfully gapped: 2
Length of query: 445
Length of database: 11,106,569
Length adjustment: 102
Effective length of query: 343
Effective length of database: 8,310,137
Effective search space: 2850376991
Effective search space used: 2850376991
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 113 (48.1 bits)