BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0248000 Os03g0248000|AK100304
         (902 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G31260.1  | chr2:13322291-13326293 REVERSE LENGTH=867          806   0.0  

>AT2G31260.1 | chr2:13322291-13326293 REVERSE LENGTH=867
          Length = 866

 Score =  806 bits (2083), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 439/888 (49%), Positives = 559/888 (62%), Gaps = 58/888 (6%)

Query: 1   MMSFRSKDRNVQPRFNWP-WRSESPLSAQLLIDIPPEIELSDYRRXXXXXXXXXXXXXXX 59
           MMS   K  NV+  F W    S S L+  LL +   EIELS+Y                 
Sbjct: 1   MMSSGHKGPNVRNFFKWQRGESSSSLTTGLLHNESHEIELSNYGGIPSPGSESPSGLLNG 60

Query: 60  XXFKDEPIADLDIFFERLYEYFCAKGLRCIVTKWIIEMLNVLFMVCCIGFFFLIVDWNAL 119
                +PIADLD+F ERLY Y+  KGL CI+ KW +E+L++ F++C  GFF L VDWN L
Sbjct: 61  ESLNVQPIADLDLFVERLYSYYRDKGLWCIIVKWAVELLSLGFIICFSGFFLLYVDWNGL 120

Query: 120 GHLKCGVEALESGEKPCDLMQVVKH-NPLVPFTFPKMITIGSMVILTTYGLINFLKFFVQ 178
            + KCG++A+ESG KPCDL++   H +PL PFT    I +G + + + Y L  FL+FF Q
Sbjct: 121 QNAKCGMDAVESGTKPCDLVKEAIHPHPLSPFTLTTAIIVGYLALFSVYWLFCFLRFFAQ 180

Query: 179 LRSTLNIRDFYCNSLKITDLEIQTISWPKIIEKVVLLQKSQKLCVVRDLSEHDIIMRIMR 238
           L+ TL+ R FY N+L +TD EI T+ W  ++EKVV LQ SQ LCVV+DLS HD++MR+MR
Sbjct: 181 LKDTLDFRHFYYNNLHVTDNEILTMPWATVLEKVVQLQSSQCLCVVKDLSAHDMVMRLMR 240

Query: 239 KENYLIGMVNKGIISFPIRPWVPGAGPTVKSHLQNRRNHLILPKALEWTLNWCIFQSMFD 298
           KENYLIGM+NKG++SFPI  W+PGAGP VKS     + HL+L K LEWTLNWCI QSMFD
Sbjct: 241 KENYLIGMLNKGLLSFPISHWIPGAGPAVKSAPDGTQYHLVLTKTLEWTLNWCILQSMFD 300

Query: 299 SKFCVRKDFLTSPAVLKKRLVFVGISMLILSPCLVIFPLVYLILRHAEEIYNHPSTASSR 358
             F VR+DF+++P  LKKRL  VG++ML+LSP LVIF LVYL LRHAE+ YNHPSTASSR
Sbjct: 301 CNFRVRRDFVSNPTTLKKRLFVVGLAMLLLSPFLVIFMLVYLFLRHAEQFYNHPSTASSR 360

Query: 359 RWSNLSRWIFREYNEVDHFFRHRMNNSAVHSLNYLKQFPTPLISIMAKFISFVSXXXXXX 418
           RWSNLS+W+FRE+NEVDH F+HR+N+S VH+  YLKQFP+P+ISI+AKF+SFVS      
Sbjct: 361 RWSNLSKWLFREFNEVDHLFKHRINSSVVHASEYLKQFPSPIISIIAKFVSFVSGGFAAV 420

Query: 419 XXXXXXXXESVLEGHIFGRNLFWYTIVFGTIAAISRKVVADELQVIDPEGAMCNVVQQTH 478
                   ES+LEGHIFGRNLFWY  VFGTI AISR  ++DEL V+DP G M  VVQ TH
Sbjct: 421 LIIIAFLEESLLEGHIFGRNLFWYAAVFGTITAISRAAISDELLVLDPVGTMSLVVQNTH 480

Query: 479 YMPKRWRGKEDSEVVRREFETLFQFTIVMLLEEMASIFISPYLLIFEVPKRVDDILRFIS 538
           YMPKRWRGKE+ + VR E ETLFQ+T +MLLEE+ASIFI+P+LL+F VPKRVDDIL+FI 
Sbjct: 481 YMPKRWRGKENKDDVRLELETLFQYTGMMLLEEIASIFITPFLLMFVVPKRVDDILQFIK 540

Query: 539 DFTIYVDGVGDVCSLSLFDFRRHGNRNYASPFDAL-KTLRSSQGKMEKSFLSFQSVYPSW 597
           DFT+ ++GVG VCS S F F  HGN  Y SP +A  +  RSSQGKMEKSFLSFQS YPSW
Sbjct: 541 DFTVDIEGVGHVCSFSAFYFENHGNIKYGSPHNATRREQRSSQGKMEKSFLSFQSSYPSW 600

Query: 598 EPNAEGKQFLTNLQKFKEKQIRQQALAQYQAMEASGFVASTRGHRDDIFHQLLPSDIHNR 657
           E ++ GKQFL+NL+ F+++++ +          A     +T     DI    L S  H  
Sbjct: 601 ESDSLGKQFLSNLRTFRDRKLHEINTRHSSPSRAWRESTNTPALYRDIPRNPLASGNHTD 660

Query: 658 AEAISPAVYNLGPLGLLDTDQRSHPYILDWYYVCHPPHLDRTEAPYFNEVFPETSENTGS 717
           +            + L+D DQR+HPY+LDWYY       +RT+ P               
Sbjct: 661 S------------MWLIDPDQRNHPYLLDWYYTSQAH--NRTDHP--------------- 691

Query: 718 AAFKASEIEEARGWDSDMVPPPRADRDEWNFNHERVRSHMDASTSSNLFHHAPVEHHDTK 777
              +A+EI  A    +D  PP    R E      R   +M+ASTS   F  + + H   +
Sbjct: 692 -IERANEILTANQNATDCWPPDLGIRGE----DSRDLLNMEASTSGQFFRESILRHDQPE 746

Query: 778 G-------NIID----WWDQAPEHST------GQQGSFLEPPEFGNRYVAGNRSSYHSGD 820
           G       + +D    WW +   HS           SF+EPP+F NRY AGN        
Sbjct: 747 GEDSYGSQHPLDGRNQWWGRG-NHSQISTAHPATTNSFIEPPDFINRYTAGN---LLDNS 802

Query: 821 VSDGSVEELEXXXXXXXXXXXXPQDLSTTRYMDDSDIEEGLNLPFADL 868
            S  S+EE +             ++LS T +MDD+DIE G++L F D+
Sbjct: 803 WSRRSIEEEDEEEEELDWEENARRNLSRTTFMDDNDIEAGIDLHFDDV 850



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.138    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 19,671,512
Number of extensions: 828605
Number of successful extensions: 1747
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1741
Number of HSP's successfully gapped: 1
Length of query: 902
Length of database: 11,106,569
Length adjustment: 108
Effective length of query: 794
Effective length of database: 8,145,641
Effective search space: 6467638954
Effective search space used: 6467638954
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 116 (49.3 bits)