BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0233900 Os03g0233900|AK064054
         (166 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G16060.1  | chr2:6982782-6983522 REVERSE LENGTH=161            230   2e-61
AT3G10520.1  | chr3:3276516-3277765 REVERSE LENGTH=159            174   3e-44

>AT2G16060.1 | chr2:6982782-6983522 REVERSE LENGTH=161
          Length = 160

 Score =  230 bits (587), Expect = 2e-61,   Method: Compositional matrix adjust.
 Identities = 106/151 (70%), Positives = 129/151 (85%), Gaps = 1/151 (0%)

Query: 12  VSFSEEQEALVLKSWAILKKDSANIALRFFLKIFEVAPSASQMFSFLRNSDVPLEKNPKL 71
           + F+EEQEALV+KSW+++KK+SA + L+ F+KIFE+AP+  +MFSFLR+S +P E+NPKL
Sbjct: 7   IVFTEEQEALVVKSWSVMKKNSAELGLKLFIKIFEIAPTTKKMFSFLRDSPIPAEQNPKL 66

Query: 72  KTHAMSVFVMTCEAAAQLRKAGKVTVRDTTLKRLGATHLKYGVGDAHFEVVKFALLDTIK 131
           K HAMSVFVM CE+A QLRK GKVTVR+TTLKRLGA+H KYGV D HFEV K+ALL+TIK
Sbjct: 67  KPHAMSVFVMCCESAVQLRKTGKVTVRETTLKRLGASHSKYGVVDEHFEVAKYALLETIK 126

Query: 132 EEVPADMWSPAMKSAWSEAYDHLVAAIKQEM 162
           E VP +MWSP MK AW +AYDHLVAAIK EM
Sbjct: 127 EAVP-EMWSPEMKVAWGQAYDHLVAAIKAEM 156



>AT3G10520.1 | chr3:3276516-3277765 REVERSE LENGTH=159
          Length = 158

 Score =  174 bits (440), Expect = 3e-44,   Method: Compositional matrix adjust.
 Identities = 88/155 (56%), Positives = 111/155 (71%), Gaps = 1/155 (0%)

Query: 12  VSFSEEQEALVLKSWAILKKDSANIALRFFLKIFEVAPSASQMFSFLRNSDVPLEKNPKL 71
           + F+E+QEALV +SW ILK+D    +L FF +I E+AP+A  +FSFLR+SD     NPKL
Sbjct: 4   IGFTEKQEALVKESWEILKQDIPKYSLHFFSQILEIAPAAKGLFSFLRDSDEVPHNNPKL 63

Query: 72  KTHAMSVFVMTCEAAAQLRKAGKVTVRDTTLKRLGATHLKYGVGDAHFEVVKFALLDTIK 131
           K HA+ VF MTCE A QLR+ GKV V DTTL+ LG+ HLK GV D HFEVVK ALL T+K
Sbjct: 64  KAHAVKVFKMTCETAIQLREEGKVVVADTTLQYLGSIHLKSGVIDPHFEVVKEALLRTLK 123

Query: 132 EEVPADMWSPAMKSAWSEAYDHLVAAIKQEMKPAE 166
           E +  + ++  ++ AWS+AYDHL  AIK EMK  E
Sbjct: 124 EGL-GEKYNEEVEGAWSQAYDHLALAIKTEMKQEE 157



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.128    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,293,181
Number of extensions: 113762
Number of successful extensions: 270
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 270
Number of HSP's successfully gapped: 2
Length of query: 166
Length of database: 11,106,569
Length adjustment: 91
Effective length of query: 75
Effective length of database: 8,611,713
Effective search space: 645878475
Effective search space used: 645878475
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 108 (46.2 bits)