BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0228500 Os03g0228500|AK101799
         (216 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G12530.1  | chr1:4269122-4270771 REVERSE LENGTH=194            110   4e-25
AT1G56420.1  | chr1:21122772-21124234 REVERSE LENGTH=184           57   6e-09

>AT1G12530.1 | chr1:4269122-4270771 REVERSE LENGTH=194
          Length = 193

 Score =  110 bits (276), Expect = 4e-25,   Method: Compositional matrix adjust.
 Identities = 71/192 (36%), Positives = 108/192 (56%), Gaps = 36/192 (18%)

Query: 7   LGMRREPSRAEVVGRAKDDGDFDALRRAIIRKVKDNEVLRSNIIAEVKQSVVINEDGSEK 66
           +GM R+ S+ EVV + KDDGDFD LR  IIR++K+NE LR+N+I+ VK+S  +   G++ 
Sbjct: 2   MGMARKISKEEVVEKLKDDGDFDRLRLKIIRRLKENEDLRNNMISVVKESTSLKRHGAQN 61

Query: 67  LKLKDLSDAIYQDIGSKIMGQISDEIWGVILSNEI---DIRGTSETVYNRMMNPE----- 118
           +K + LSDAI++++GSK++ Q+SD +WG+I S +    +IR T ++VY  + NP      
Sbjct: 62  MKTRQLSDAIFEEVGSKMLSQLSDGLWGIIRSEDGMKNEIRETVQSVYATLSNPRGEKRG 121

Query: 119 ------QQQDPPSKKLKRN---------AKEEQVSPA-------KASTSV------AVQL 150
                 + + P  KK  R           K+E +  A         S  V      A   
Sbjct: 122 TSAREMEHKIPTPKKEARTDFNTSPASKQKQELIKGAVEDNKGEACSDDVQDNKGEAYND 181

Query: 151 EDDDPEEPPGFG 162
           +++DPE PPGFG
Sbjct: 182 DEEDPELPPGFG 193



>AT1G56420.1 | chr1:21122772-21124234 REVERSE LENGTH=184
          Length = 183

 Score = 57.0 bits (136), Expect = 6e-09,   Method: Compositional matrix adjust.
 Identities = 35/108 (32%), Positives = 61/108 (56%), Gaps = 1/108 (0%)

Query: 17  EVVGRAKDDGDFDALRRAIIRKVKDNEVLRSNIIAEVKQSVVINEDGSEKLKLKDLSDAI 76
           +V+    +DG  DALR  II ++K NE L+S  I   ++S V+N  G+EK   ++L DA+
Sbjct: 11  DVLEHLMNDGTIDALRLRIINQLKANEELKSTTIKMAEESKVLNTPGAEKQTKRELFDAL 70

Query: 77  YQDIGSKIMGQISDEIWGVILSNEIDIRGTSETVYNRMMNPEQQQDPP 124
            Q++   ++ + S  +W +I+  +   +  +ETV  R+      Q+PP
Sbjct: 71  RQELEGPVLEKASKSVWDLIIEKDGLGKEINETV-ERVFCHLSGQEPP 117



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.309    0.128    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,422,460
Number of extensions: 184300
Number of successful extensions: 719
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 718
Number of HSP's successfully gapped: 2
Length of query: 216
Length of database: 11,106,569
Length adjustment: 94
Effective length of query: 122
Effective length of database: 8,529,465
Effective search space: 1040594730
Effective search space used: 1040594730
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 109 (46.6 bits)