BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0226200 Os03g0226200|AK121522
         (169 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G16060.1  | chr2:6982782-6983522 REVERSE LENGTH=161            235   1e-62
AT3G10520.1  | chr3:3276516-3277765 REVERSE LENGTH=159            166   4e-42

>AT2G16060.1 | chr2:6982782-6983522 REVERSE LENGTH=161
          Length = 160

 Score =  235 bits (599), Expect = 1e-62,   Method: Compositional matrix adjust.
 Identities = 109/155 (70%), Positives = 132/155 (85%), Gaps = 1/155 (0%)

Query: 11  SGGAVSFSEEQEALVLKSWAIMKKDSANIGLRFFLKIFEVAPSASQMFSFLRNSDVPLEK 70
           S G + F+EEQEALV+KSW++MKK+SA +GL+ F+KIFE+AP+  +MFSFLR+S +P E+
Sbjct: 3   SEGKIVFTEEQEALVVKSWSVMKKNSAELGLKLFIKIFEIAPTTKKMFSFLRDSPIPAEQ 62

Query: 71  NPKLKTHAMSVFVMTCEAAAQLRKAGKVTVRDTTLKRLGATHFKYGVGDAHFEVTRFALL 130
           NPKLK HAMSVFVM CE+A QLRK GKVTVR+TTLKRLGA+H KYGV D HFEV ++ALL
Sbjct: 63  NPKLKPHAMSVFVMCCESAVQLRKTGKVTVRETTLKRLGASHSKYGVVDEHFEVAKYALL 122

Query: 131 ETIKEAVPVDMWSPAMKSAWSEAYNQLVAAIKQEM 165
           ETIKEAVP +MWSP MK AW +AY+ LVAAIK EM
Sbjct: 123 ETIKEAVP-EMWSPEMKVAWGQAYDHLVAAIKAEM 156



>AT3G10520.1 | chr3:3276516-3277765 REVERSE LENGTH=159
          Length = 158

 Score =  166 bits (421), Expect = 4e-42,   Method: Compositional matrix adjust.
 Identities = 83/157 (52%), Positives = 108/157 (68%), Gaps = 1/157 (0%)

Query: 13  GAVSFSEEQEALVLKSWAIMKKDSANIGLRFFLKIFEVAPSASQMFSFLRNSDVPLEKNP 72
           G + F+E+QEALV +SW I+K+D     L FF +I E+AP+A  +FSFLR+SD     NP
Sbjct: 2   GEIGFTEKQEALVKESWEILKQDIPKYSLHFFSQILEIAPAAKGLFSFLRDSDEVPHNNP 61

Query: 73  KLKTHAMSVFVMTCEAAAQLRKAGKVTVRDTTLKRLGATHFKYGVGDAHFEVTRFALLET 132
           KLK HA+ VF MTCE A QLR+ GKV V DTTL+ LG+ H K GV D HFEV + ALL T
Sbjct: 62  KLKAHAVKVFKMTCETAIQLREEGKVVVADTTLQYLGSIHLKSGVIDPHFEVVKEALLRT 121

Query: 133 IKEAVPVDMWSPAMKSAWSEAYNQLVAAIKQEMKPAE 169
           +KE +  + ++  ++ AWS+AY+ L  AIK EMK  E
Sbjct: 122 LKEGLG-EKYNEEVEGAWSQAYDHLALAIKTEMKQEE 157



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.129    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,355,316
Number of extensions: 114948
Number of successful extensions: 271
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 269
Number of HSP's successfully gapped: 2
Length of query: 169
Length of database: 11,106,569
Length adjustment: 91
Effective length of query: 78
Effective length of database: 8,611,713
Effective search space: 671713614
Effective search space used: 671713614
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 108 (46.2 bits)