BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0221200 Os03g0221200|AK068002
         (282 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G25900.1  | chr3:9480964-9482684 FORWARD LENGTH=327            396   e-111
AT3G63250.1  | chr3:23370575-23372587 REVERSE LENGTH=334          328   3e-90
AT3G22740.1  | chr3:8033200-8035710 REVERSE LENGTH=348            313   5e-86

>AT3G25900.1 | chr3:9480964-9482684 FORWARD LENGTH=327
          Length = 326

 Score =  396 bits (1018), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 182/277 (65%), Positives = 225/277 (81%), Gaps = 1/277 (0%)

Query: 5   VEEIVRRAGGCAVIDGGFATQLEALGADINDPLWSAACLITKPHLIKEVHMQYLEAGADV 64
           +E+++++ GGCAV+DGGFATQLE  GA INDPLWSA  LI  P LIK VHM+YLEAGAD+
Sbjct: 10  LEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHMEYLEAGADI 69

Query: 65  IISSSYQATIPGFLARGMLLEEAEGLLRRSIELALEARDEFWKSTLRKSKPVYNRALVAA 124
           +++SSYQATIPGFL+RG+ +EE+E LL++S+ELA+EARD FW+   + S   YNRALVAA
Sbjct: 70  VVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGHSYNRALVAA 129

Query: 125 SIGSYGAYLADGSEYSGSYGEDITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNKMEAQA 184
           SIGSYGAYLADGSEYSG YGE+++ +KLKDFHRRRLQVL  AGPDL+AFE IPNK+EAQA
Sbjct: 130 SIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQA 189

Query: 185 XXXXXXXXNIQVPSWICFSSVDGKNLCSGESFAECLQFLNASDKVTIVGVNCTPPQFIEG 244
                    +Q+P+WICF+SVDG+   SGESF ECL+ LN S+ +  VG+NC PPQFIE 
Sbjct: 190 CVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGINCAPPQFIEN 249

Query: 245 IIRELKKQTKKAIAVYPNSGEIWDGRAKRWLVSTTCY 281
           +IR+  K TKKAI VYPNSGE+WDG+AK+WL S  C+
Sbjct: 250 LIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPS-QCF 285



>AT3G63250.1 | chr3:23370575-23372587 REVERSE LENGTH=334
          Length = 333

 Score =  328 bits (840), Expect = 3e-90,   Method: Compositional matrix adjust.
 Identities = 157/284 (55%), Positives = 204/284 (71%), Gaps = 13/284 (4%)

Query: 4   AVEEIVRRAGGCAVIDGGFATQLEALGADINDPLWSAACLITKPHLIKEVHMQYLEAGAD 63
           ++++ +++ GG AVIDGG AT+ E  GAD+NDPLWSA CL+T PHLI  VH+ YLEAGAD
Sbjct: 8   SMKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGAD 67

Query: 64  VIISSSYQATIPGFLARGMLLEEAEGLLRRSIELALEARDEFWK---------STLRKSK 114
           +I S+SYQATI GF A+G   EE+E LL++S+E+A EAR+ ++            + K +
Sbjct: 68  IISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKR 127

Query: 115 PVYNRALVAASIGSYGAYLADGSEYSGSYGEDITAEKLKDFHRRRLQVLASAGPDLIAFE 174
           P+    LVAAS+GSYGAYLADGSEYSG YG+ IT EKLKDFHRRRLQVLA +G DLIAFE
Sbjct: 128 PI----LVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFE 183

Query: 175 AIPNKMEAQAXXXXXXXXNIQVPSWICFSSVDGKNLCSGESFAECLQFLNASDKVTIVGV 234
            IPNK+EAQA        ++++P W  F+S DG N+ SG+S  EC+      +KV  VG+
Sbjct: 184 TIPNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGI 243

Query: 235 NCTPPQFIEGIIRELKKQTKKAIAVYPNSGEIWDGRAKRWLVST 278
           NCTPP+FIEG++ E++K T K I VYPNSGE +D   K W+ +T
Sbjct: 244 NCTPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENT 287



>AT3G22740.1 | chr3:8033200-8035710 REVERSE LENGTH=348
          Length = 347

 Score =  313 bits (803), Expect = 5e-86,   Method: Compositional matrix adjust.
 Identities = 146/278 (52%), Positives = 200/278 (71%), Gaps = 7/278 (2%)

Query: 7   EIVRRAGGCAVIDGGFATQLEALGADINDPLWSAACLITKPHLIKEVHMQYLEAGADVII 66
           + + + GG AV+DGGFAT+L+  GADINDPLWSA CLIT PHL+ +VH+ YLE+GA++II
Sbjct: 15  DFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYLESGANIII 74

Query: 67  SSSYQATIPGFLARGMLLEEAEGLLRRSIELALEARDEFWKSTLRKS-------KPVYNR 119
           ++SYQATI GF+A+G+ + EAE LLRRS+E+  EAR+ F+    + S       K     
Sbjct: 75  TASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAYAGKASRRP 134

Query: 120 ALVAASIGSYGAYLADGSEYSGSYGEDITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNK 179
            LVAAS+GSYGAYLADGSEYSG YG+ ++ E LKDFHRRR+Q+LA +G DLIAFE IPNK
Sbjct: 135 ILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADLIAFETIPNK 194

Query: 180 MEAQAXXXXXXXXNIQVPSWICFSSVDGKNLCSGESFAECLQFLNASDKVTIVGVNCTPP 239
           +EA+A        +I +P+W  F+S DG ++  G+S  EC +  ++   V  +G+NCT P
Sbjct: 195 LEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVVAIGINCTAP 254

Query: 240 QFIEGIIRELKKQTKKAIAVYPNSGEIWDGRAKRWLVS 277
           ++I  +I  L++ T+K I VYPNSGE++DG  K+W+ S
Sbjct: 255 RYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKS 292



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.134    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,011,293
Number of extensions: 233911
Number of successful extensions: 578
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 576
Number of HSP's successfully gapped: 3
Length of query: 282
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 185
Effective length of database: 8,447,217
Effective search space: 1562735145
Effective search space used: 1562735145
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 111 (47.4 bits)