BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0187400 Os03g0187400|AK100103
(200 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G60520.1 | chr3:22361751-22362467 REVERSE LENGTH=130 54 5e-08
AT1G02070.1 | chr1:370445-370947 REVERSE LENGTH=132 53 9e-08
>AT3G60520.1 | chr3:22361751-22362467 REVERSE LENGTH=130
Length = 129
Score = 53.9 bits (128), Expect = 5e-08, Method: Compositional matrix adjust.
Identities = 23/46 (50%), Positives = 29/46 (63%), Gaps = 1/46 (2%)
Query: 25 VCCMCGDRGLLPELFRCAACAVRSQHTYCSERYP-KAESYGTCNWC 69
VCCMCGD G +LF C+ C R QH+YCS Y +A+ C+WC
Sbjct: 8 VCCMCGDVGFFDKLFHCSKCLNRFQHSYCSSYYKEQADPIKICDWC 53
>AT1G02070.1 | chr1:370445-370947 REVERSE LENGTH=132
Length = 131
Score = 53.1 bits (126), Expect = 9e-08, Method: Compositional matrix adjust.
Identities = 25/51 (49%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Query: 25 VCCMCGDRGLLPELFRCAACAVRSQHTYCSERYPK-AESYGTCNWCLRADQ 74
VCCMCGD G +LF C C R QH+YCS Y + AE C+WC D+
Sbjct: 4 VCCMCGDVGFSDKLFSCGHCRCRFQHSYCSNYYGQFAEPTEICDWCRSDDR 54
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.325 0.135 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,263,490
Number of extensions: 50450
Number of successful extensions: 135
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 135
Number of HSP's successfully gapped: 2
Length of query: 200
Length of database: 11,106,569
Length adjustment: 94
Effective length of query: 106
Effective length of database: 8,529,465
Effective search space: 904123290
Effective search space used: 904123290
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 109 (46.6 bits)