BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0186500 Os03g0186500|AK111688
         (1308 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G66820.1  | chr5:26689097-26691110 FORWARD LENGTH=520           65   4e-10

>AT5G66820.1 | chr5:26689097-26691110 FORWARD LENGTH=520
          Length = 519

 Score = 64.7 bits (156), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 47/161 (29%), Positives = 72/161 (44%), Gaps = 38/161 (23%)

Query: 767 SSMVLIRSNISGMEWLPLQAHGGRQSIVLLEESEPVWKLEFNLISDIQNKSALSKSEFTS 826
           SS  LIR N+SG+ WL L+               PV  +EF   +   +           
Sbjct: 120 SSSALIRKNLSGVVWLSLK---------------PVHIIEFQPFTGFFH---------IG 155

Query: 827 PLCGQHLSKEIHVKNSGDLPLQVTKVKISGADCAVDGFSVDN-CKGFSLAPSESIRMLVS 885
             C + +SKE++ K +     +   + +SG  C  +GF V++ C+GFSL P +SI+ L  
Sbjct: 156 DTCYEPMSKELYTKKT----TRELSITVSGKQCGGNGFMVNHPCEGFSLEPGDSIKFLFF 211

Query: 886 FKADFSSAMVQRDLELAMTTGIFIIPMTANIPVCMLDQCRK 926
           +         Q +L  A    +F +PM A  PV ML   +K
Sbjct: 212 Y---------QSELSWASGVAVFAVPMKATAPVLMLSLYKK 243



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.317    0.132    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 24,691,737
Number of extensions: 979696
Number of successful extensions: 2368
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 2367
Number of HSP's successfully gapped: 1
Length of query: 1308
Length of database: 11,106,569
Length adjustment: 110
Effective length of query: 1198
Effective length of database: 8,090,809
Effective search space: 9692789182
Effective search space used: 9692789182
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 118 (50.1 bits)