BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0178400 Os03g0178400|AK108257
(328 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G21950.1 | chr5:7254067-7255866 REVERSE LENGTH=309 196 1e-50
AT4G33180.1 | chr4:16000282-16002055 FORWARD LENGTH=308 172 2e-43
AT1G17430.1 | chr1:5982310-5983919 FORWARD LENGTH=333 138 5e-33
AT1G78210.1 | chr1:29422820-29424294 REVERSE LENGTH=315 135 2e-32
AT1G72620.1 | chr1:27341079-27342401 FORWARD LENGTH=336 125 4e-29
AT5G09430.1 | chr5:2932162-2933362 FORWARD LENGTH=312 119 2e-27
AT4G39955.1 | chr4:18530573-18532088 FORWARD LENGTH=329 113 1e-25
AT4G36610.1 | chr4:17265545-17267274 REVERSE LENGTH=318 102 4e-22
AT2G18360.1 | chr2:7976848-7979221 REVERSE LENGTH=314 100 2e-21
AT1G13820.1 | chr1:4735622-4737617 FORWARD LENGTH=340 51 9e-07
>AT5G21950.1 | chr5:7254067-7255866 REVERSE LENGTH=309
Length = 308
Score = 196 bits (499), Expect = 1e-50, Method: Compositional matrix adjust.
Identities = 116/324 (35%), Positives = 177/324 (54%), Gaps = 28/324 (8%)
Query: 5 LSLVPLLDYFARREFAAAGLSPGAVTLPYPGDGGEATCTVHYW-----ASTGEPLLPPLL 59
LS+ ++ RR F++AGLS +++ ++ T+ +W +S+ P LL
Sbjct: 6 LSVARFVEALLRRRFSSAGLSLQTLSI-------DSETTIQFWGPPPSSSSENTQKPSLL 58
Query: 60 LVHGFGPRATWQWRCQVGPLSRHFHLIVPDLIXXXXXXXXXXXXXXXXXEATQXXXXXXX 119
L+HGFGP A WQW QV PLS F L VPDL+ ++
Sbjct: 59 LLHGFGPSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGE-------NRSEMFQALCM 111
Query: 120 XXXXPGMKGRRVAVAGTSYGGFVAYWLARTAGAGRVGPVVIASSDLLKTAADDRGFLKRA 179
++ R +V GTSYGGFVAY +A+ +V VV+ASS + +D+ F+ RA
Sbjct: 112 GKLMEKLEVERFSVVGTSYGGFVAYNMAKMF-PEKVEKVVLASSGVNLRRSDNEAFIARA 170
Query: 180 GDGWSGVEEVLLPAEPAAMRKLLEMASCRPPPPVLMPDFILRDFIQKLFTENREQLIQLF 239
++EV+LPA +R+ M S + V PDF+L DF QK+++E RE+ +L
Sbjct: 171 K--CHRIKEVMLPASATDLRRFSGMVSSKRLDYV--PDFVLNDFCQKMYSEKREEKAELL 226
Query: 240 KGITVGTD-KFPVTPISQEVLIVWGEQDQLFPVEKAYAVQSSLDGKARVEIISKTGHAPQ 298
+G+++G D K V+PI Q+V+++WGEQDQ+FP++ A+ ++ L KA +++I KT H PQ
Sbjct: 227 EGLSIGKDDKTNVSPIQQDVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQ 286
Query: 299 LEDPTRFNKILLDFLLATHKPDPS 322
E FN ++ FLL P PS
Sbjct: 287 TEKSKEFNGFVMSFLLP---PSPS 307
>AT4G33180.1 | chr4:16000282-16002055 FORWARD LENGTH=308
Length = 307
Score = 172 bits (436), Expect = 2e-43, Method: Compositional matrix adjust.
Identities = 108/320 (33%), Positives = 164/320 (51%), Gaps = 25/320 (7%)
Query: 1 MGASLSLVPLLDYFARREFAAAGLSPGAVTLPYPGDGGEATCTVHYWA-----STGEPLL 55
+ + LS LL+ + RR AAGL+ +++ ++ T+H+W +
Sbjct: 3 LSSYLSPTRLLEGYLRRCLRAAGLTSQTLSI-------DSETTIHFWGPPPLDHRSDDDR 55
Query: 56 PPLLLVHGFGPRATWQWRCQVGPLS-RHFHLIVPDLIXXXXXXXXXXXXXXXXXEATQXX 114
P +LL+HGFGP + WQWR Q+ S F + PDL+ T+
Sbjct: 56 PVMLLLHGFGPSSMWQWRRQMQAFSPSAFRVYSPDLVFFGDSTSSST-------NRTEVF 108
Query: 115 XXXXXXXXXPGMKGRRVAVAGTSYGGFVAYWLARTAGAGRVGPVVIASSDLLKTAADDRG 174
+ + VAGTSYGGFVAY +A+ +V VVIASS + D
Sbjct: 109 QAECMAKLMAKIGIGKYNVAGTSYGGFVAYHMAKM-WPEKVEKVVIASSGINMRKCDGES 167
Query: 175 FLKRAGDGWSGVEEVLLPAEPAAMRKLLEMASCRPPPPVLMPDFILRDFIQKLFTENREQ 234
L+R+ +E+V+LP+ R L+ +AS + PD + D I L+ +NR++
Sbjct: 168 LLQRSN--CECIEKVMLPSTATEFRTLMALASSWRLVR-MFPDALWNDVINNLYKKNRKE 224
Query: 235 LIQLFKGITVG-TDKFPVTPISQEVLIVWGEQDQLFPVEKAYAVQSSLDGKARVEIISKT 293
I+L KG+T G ++ + +SQEVLIVWG++DQ+FPV+ AY ++ L K ++EII T
Sbjct: 225 KIELLKGVTFGRSENLNIDSLSQEVLIVWGDKDQIFPVKMAYELKEILGDKTKLEIIDNT 284
Query: 294 GHAPQLEDPTRFNKILLDFL 313
H PQ+E FN I+L FL
Sbjct: 285 SHVPQIECAQEFNNIVLRFL 304
>AT1G17430.1 | chr1:5982310-5983919 FORWARD LENGTH=333
Length = 332
Score = 138 bits (347), Expect = 5e-33, Method: Compositional matrix adjust.
Identities = 101/312 (32%), Positives = 157/312 (50%), Gaps = 17/312 (5%)
Query: 7 LVPLLDYFARREFAA-AGLSPGAVTLPYPGDGGEATCTVHYWASTGEPL-LPPLLLVHGF 64
L+ + D F F L P V L D GE T VH+W S + L+++HG+
Sbjct: 34 LIKIADSFLSLYFLVFCDLRPVTVDL----DDGETT--VHFWISGHRRISRQNLVMLHGY 87
Query: 65 GPRATWQWRCQVGPLSRHFHLIVPDLIXXXXXXXXXXXXXXXXXEATQXXXXXXXXXXXP 124
G + WQ+ QV LS+ F+L +PDL+ +A
Sbjct: 88 GGNSKWQFVHQVSDLSKSFNLFIPDLVFFGKSYSKNRDRSVEI-QARSVVGGLKKLGCVE 146
Query: 125 GMKGRRVAVAGTSYGGFVAYWLARTAGAGRVGPVVIASSDLLKTAADDRGFLKRAGDGWS 184
G G +++ SYGGFVAY +A A V +VI SS + T LK+ G S
Sbjct: 147 G--GGGISIYSISYGGFVAYKMAEIWPA-MVEKLVIVSSGVGFTQQQKTAELKKHGGDCS 203
Query: 185 GVEEVLLPAEPAAMRKLLEMASCRPPPPV-LMPDFILRDFIQKLFTENREQLIQLFKGIT 243
++L+P P +R L++++ V +PDF L FI ++ +NR++L++L K +
Sbjct: 204 ---KILVPKTPMDLRLLIKISMNTGLTFVDWVPDFFLSQFIAVMYEKNRQELLELAKNLL 260
Query: 244 VGTDKFPVTPISQEVLIVWGEQDQLFPVEKAYAVQSSLDGKARVEIISKTGHAPQLEDPT 303
++ + ISQ+ LIVWG++D++FP+E AY +Q L +R+EII +TGHA +E PT
Sbjct: 261 EREEEPELPVISQKTLIVWGDKDKVFPLEHAYRLQRHLQS-SRLEIIKETGHAVNIEAPT 319
Query: 304 RFNKILLDFLLA 315
N + F+L+
Sbjct: 320 TLNNFITSFVLS 331
>AT1G78210.1 | chr1:29422820-29424294 REVERSE LENGTH=315
Length = 314
Score = 135 bits (341), Expect = 2e-32, Method: Compositional matrix adjust.
Identities = 98/329 (29%), Positives = 148/329 (44%), Gaps = 21/329 (6%)
Query: 1 MGASLSLVPLLDYFARREFAAAGLSPGAVTLPYPGDGGEATCTVHYWASTGEPLLPP--- 57
M SL L+ + F +GL P + L DG V++W S +P P
Sbjct: 1 MTGCFSLSEALERTYKSGFKRSGLRPVTIDLK---DG----TVVNFWVSKTKPESKPKPN 53
Query: 58 LLLVHGFGPRATWQWRCQVGPLSRHFHLIVPDLIXXXXXXXXXXXXXXXXXEATQXXXXX 117
LLL+HG G A WQW LSR+F+L +PDL+ E +
Sbjct: 54 LLLIHGLGATAIWQWYDVARRLSRYFNLYIPDLVFFGGSSTTRP-------ERSDIFQAQ 106
Query: 118 XXXXXXPGMKGRRVAVAGTSYGGFVAYWLARTAGAGRVGPVVIASSDLLKTAADDRGFLK 177
++ ++ G SYGGFV Y +A + A V VVI + + D + +
Sbjct: 107 TLMRALEAQSVKKFSLVGLSYGGFVGYRMA-SMYADAVEKVVICCAAVCVEEKDMKAGVF 165
Query: 178 RAGDGWSGVEEVLLPAEPAAMRKLLEMASCRPPPPVLMPDFILRDFIQKLFT-ENREQLI 236
+ D ++L+P +R+L+ +P L+P +L DFI+ T +N E+
Sbjct: 166 KVSD-LDEASKILVPESVKKLRELMGYIFYKPALARLVPTCLLHDFIEHALTRDNMEEKR 224
Query: 237 QLFKGITVGTDKFPVTPISQEVLIVWGEQDQLFPVEKAYAVQSSLDGKARVEIISKTGHA 296
+L K I + + Q LI+WGE DQ+FP+E ++ + ++ II +TGH
Sbjct: 225 ELIKAIPKDRIISEIPKLKQPTLIIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHI 284
Query: 297 PQLEDPTRFNKILLDFLLATHKPD-PSSN 324
E P +F K+L FLL T KP P SN
Sbjct: 285 FNFEKPKKFIKLLKSFLLETSKPQIPVSN 313
>AT1G72620.1 | chr1:27341079-27342401 FORWARD LENGTH=336
Length = 335
Score = 125 bits (313), Expect = 4e-29, Method: Compositional matrix adjust.
Identities = 87/293 (29%), Positives = 140/293 (47%), Gaps = 24/293 (8%)
Query: 32 PYPGDGGEATCTVHYWASTGEPL-LPPLLLVHGFGPRATWQWRCQVGPLSRHFHLIVPDL 90
P D + T+H+W S + P L+++HG+G + WQ+ QV LS+ F+L +PDL
Sbjct: 58 PITVDLNDGETTLHFWISGHRKINRPNLVMLHGYGGNSKWQFIHQVSDLSKSFNLFIPDL 117
Query: 91 IXXXXXXXXXXXXXXXXXEATQXXXXXXXXXXXPGMK-----GRRVAVAGTSYGGFVAYW 145
+ T G+K ++V SYGGFVAY
Sbjct: 118 VFFGKSYS----------RNTDRTIEFQARSIVGGLKRLGCGDGDLSVYSISYGGFVAYR 167
Query: 146 LARTAGAGRVGPVVIASSDLLKTAADDRGFLKRAGDGWSGVEEVLLPAEPAAMRKLLEMA 205
+A+ + +VI SS + T +K+ G V E+L+P+ P +R LL
Sbjct: 168 IAKIW-PEMIEKLVIVSSGVGFTQQQKMTEMKKHG---GDVSEILVPSNPRDLR-LLVKV 222
Query: 206 SCRPPPPVL--MPDFILRDFIQKLFTENREQLIQLFKGITVGTDKFPVTPISQEVLIVWG 263
S L +PDFIL FI ++ NR++L+ L K + ++ + ISQ LIVWG
Sbjct: 223 SMNTGIRFLDWVPDFILSQFIAVMYETNRQELVDLAKNLLEREEEPELFSISQRTLIVWG 282
Query: 264 EQDQLFPVEKAYAVQSSLDGKARVEIISKTGHAPQLEDPTRFNKILLDFLLAT 316
++D +FP+E +Q L + +E++ + GH +E PT N +++ F+L
Sbjct: 283 DKDNVFPLEHGRRLQRHLPNSS-LEVLKEIGHGVNIEAPTTLNNLIISFVLGV 334
>AT5G09430.1 | chr5:2932162-2933362 FORWARD LENGTH=312
Length = 311
Score = 119 bits (299), Expect = 2e-27, Method: Compositional matrix adjust.
Identities = 90/309 (29%), Positives = 135/309 (43%), Gaps = 20/309 (6%)
Query: 12 DYFARREFAAAGLSPGAVTLPYPGDGGEATCTVHYWA-STGEPLLPPLLLVHGFGPRATW 70
D+ R+ FA AGL +VT A+ +H W + P LLL+HGFG A W
Sbjct: 18 DWLFRQSFANAGLR--SVTTDLSHGNSIASTAMHCWIPKSPNRSKPNLLLLHGFGANAMW 75
Query: 71 QWRCQVGPLSRHFHLIVPDLIXXXXXXXXXXXXXXXXXEATQXXXXXXXXXXXPGMKGRR 130
Q+ + + F++ VPDL+ T+ +R
Sbjct: 76 QYGEHLRAFTGRFNVYVPDLLFFGLSSTSEP-------NRTESFQARCLMRLMEAHGVQR 128
Query: 131 VAVAGTSYGGFVAYWLARTAGAGRVGPVVIASSDLLKTAADDRGFLKRAGDGWSGVEE-- 188
+ + G SYGGFV Y LA V+ + L+ + G K +EE
Sbjct: 129 MNIVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPN-----LEEAT 183
Query: 189 -VLLPAEPAAMRKLLEMASCRPPPPVLMPDFILRDFIQKLFTENREQLIQLFKGITVGTD 247
+L+P P +++L+ + +P V P F L DFI + TE E+ L K I
Sbjct: 184 GILIPQTPEKLKELIRFSFVKPIKGV--PSFFLWDFIDVMCTEFVEEKRDLIKSILKDRR 241
Query: 248 KFPVTPISQEVLIVWGEQDQLFPVEKAYAVQSSLDGKARVEIISKTGHAPQLEDPTRFNK 307
+ I Q+ LI+WGE+DQ+FP+E Y ++ + A + +I K GHA LE F K
Sbjct: 242 LSDLPRIKQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVVIKKAGHAVNLEKSKEFVK 301
Query: 308 ILLDFLLAT 316
L FL+ +
Sbjct: 302 HLKSFLIDS 310
>AT4G39955.1 | chr4:18530573-18532088 FORWARD LENGTH=329
Length = 328
Score = 113 bits (282), Expect = 1e-25, Method: Compositional matrix adjust.
Identities = 89/315 (28%), Positives = 139/315 (44%), Gaps = 20/315 (6%)
Query: 1 MGASLSLVPLLDYFARREFAAAGLSPGAVTLPYPGDGGEATCTVHYWAS-TGEPLLPPLL 59
M + S V + + F+ AGL L GDG H W T P LL
Sbjct: 1 MASCFSYVSSRNKCYQYSFSRAGLRSSTSDL---GDGT----VFHCWIPLTHIHTKPTLL 53
Query: 60 LVHGFGPRATWQWRCQVGPLSRHFHLIVPDLIXXXXXXXXXXXXXXXXXEATQXXXXXXX 119
L+HG G A WQW + F++ VPDLI + ++
Sbjct: 54 LLHGIGANAMWQWDRFIDRFIPRFNVYVPDLIFFGDSYTTRP-------DRSESFQATCV 106
Query: 120 XXXXPGMKGRRVAVAGTSYGGFVAYWLARTAGAGRVGPVVIASSDLLKTAADDRGFLKRA 179
R + VAG SYGGFVAY LA V+I + L+ + G K
Sbjct: 107 MKAMDAYGVRTMTVAGLSYGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVK 166
Query: 180 GDGWSGVEEVLLPAEPAAMRKLLEMASCRPPPPVLMPDFILRDFIQKLFTENREQLIQLF 239
+ VL P P+ +R+LL+++ +PP + +P D+I + + ++ +L
Sbjct: 167 SPEEAAA--VLFPQSPSMLRRLLQLSFYKPP--IWIPSCFAMDYIHVMCKDYLQERKELV 222
Query: 240 KGITVGTDKFPVTPISQEVLIVWGEQDQLFPVEKAYAVQSSL-DGKARVEIISKTGHAPQ 298
+ + G + I+Q L++WGE+DQ+FPVE A+ ++ L + +A++ ++ KTGHA
Sbjct: 223 EALHKGRRFANLPKITQPTLMIWGEEDQVFPVELAHRLKRYLGEDRAQLVLLKKTGHAIN 282
Query: 299 LEDPTRFNKILLDFL 313
E P K + FL
Sbjct: 283 EEKPKEMYKHMKSFL 297
>AT4G36610.1 | chr4:17265545-17267274 REVERSE LENGTH=318
Length = 317
Score = 102 bits (253), Expect = 4e-22, Method: Compositional matrix adjust.
Identities = 73/269 (27%), Positives = 122/269 (45%), Gaps = 26/269 (9%)
Query: 58 LLLVHGFGPRATWQWRCQVGPLSRHFHLIVPDLIXXXXXXXXXXXXXXXXXEATQXXXXX 117
+LL+HGF W+ QVG LS+ + + +PDL+ T
Sbjct: 63 VLLIHGFAGEGIVTWQFQVGALSKKYSVYIPDLLFFGGSY-------------TDNSDRS 109
Query: 118 XXXXXXPGMKGRRV------AVAGTSYGGFVAYWLARTAGAGRVGPVVIASSDLLKTAAD 171
+KG R+ G SYGG VA+ +A A V +V++ S T
Sbjct: 110 PAFQADCLVKGLRILGVDKFVPVGFSYGGMVAFKIAE-AYPDMVRAIVVSGSIPTMTDTI 168
Query: 172 DRGFLKRAGDGWSGVEEVLLPAEPAAMRKLLEMASCRPPPPVLMPDFILRDFIQKLFTEN 231
+ L R G +S ++LLP ++ L +A +P + P + +D+I+ +F N
Sbjct: 169 NEASLNRLG--FSSSTDLLLPTSVTGLKALFTIAVHKP---LWFPKRLFKDYIEVMFN-N 222
Query: 232 REQLIQLFKGITVGTDKFPVTPISQEVLIVWGEQDQLFPVEKAYAVQSSLDGKARVEIIS 291
R++ +L + + V + + +++ +WGE DQ+F +E A ++ + A +E I
Sbjct: 223 RKERAELLEAVVVSNKEAQIPHFPRKIHFLWGESDQIFDLELARDMKEQIGENATIESIK 282
Query: 292 KTGHAPQLEDPTRFNKILLDFLLATHKPD 320
K GH QLE P +N+ L FL + H D
Sbjct: 283 KAGHLVQLERPCVYNRRLKKFLASIHSED 311
>AT2G18360.1 | chr2:7976848-7979221 REVERSE LENGTH=314
Length = 313
Score = 99.8 bits (247), Expect = 2e-21, Method: Compositional matrix adjust.
Identities = 67/269 (24%), Positives = 121/269 (44%), Gaps = 14/269 (5%)
Query: 52 EPLLPPLLLVHGFGPRATWQWRCQVGPLSRHFHLIVPDLIXXXXXXXXXXXXXXXXXEAT 111
+P P LL +HGF W+ QVG L++ + + +PDL+ + +
Sbjct: 59 KPTKPVLLFIHGFAAEGIVTWQFQVGSLAKKYSVYIPDLLFFGGSYSDNA-------DRS 111
Query: 112 QXXXXXXXXXXXPGMKGRRVAVAGTSYGGFVAYWLARTAGAGRVGPVVIASSDLLKTAAD 171
+ + + G SYGG VA+ +A V +V++ S L T
Sbjct: 112 PAFQAHCLVKSLRILGIEKFTLVGFSYGGMVAFKIAEEY-PEMVQAMVVSGSILAMTDTI 170
Query: 172 DRGFLKRAGDGWSGVEEVLLPAEPAAMRKLLEMASCRPPPPVLMPDFILRDFIQKLFTEN 231
L + G + ++LLP ++ L +A +P + P + +DFI+ + T N
Sbjct: 171 SESNLNQLG--FKSSADLLLPTSVKGLKTLFTLAVHKP---MWFPKRLFKDFIEVMIT-N 224
Query: 232 REQLIQLFKGITVGTDKFPVTPISQEVLIVWGEQDQLFPVEKAYAVQSSLDGKARVEIIS 291
R++ +L + + + + Q++ ++WGE DQ+F +E A +++ L A +E I
Sbjct: 225 RKERAELLEALVISNKDVTIPRFQQKIHLLWGESDQIFNLEFAKSMKEQLGENATMESIK 284
Query: 292 KTGHAPQLEDPTRFNKILLDFLLATHKPD 320
K GH LE P +N+ L FL + + +
Sbjct: 285 KAGHLAHLERPCVYNRRLKKFLASVYSEN 313
>AT1G13820.1 | chr1:4735622-4737617 FORWARD LENGTH=340
Length = 339
Score = 50.8 bits (120), Expect = 9e-07, Method: Compositional matrix adjust.
Identities = 24/68 (35%), Positives = 39/68 (57%), Gaps = 1/68 (1%)
Query: 251 VTPISQEVLIVWGEQDQLFPVEKAYAVQSSLDGKARVEIISKTGHAPQLEDPTRFNKILL 310
+ +SQ+ LI+WGE DQ+ + A+ + L ARV+ IS GH P +E P K++
Sbjct: 265 IKKVSQKTLILWGEDDQIISNKLAWRLHGELSN-ARVKQISNCGHLPHVEKPAAVTKLIA 323
Query: 311 DFLLATHK 318
+F+ T +
Sbjct: 324 EFVRETCR 331
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.320 0.138 0.430
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,971,330
Number of extensions: 283216
Number of successful extensions: 606
Number of sequences better than 1.0e-05: 10
Number of HSP's gapped: 588
Number of HSP's successfully gapped: 13
Length of query: 328
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 229
Effective length of database: 8,392,385
Effective search space: 1921856165
Effective search space used: 1921856165
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 112 (47.8 bits)