BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0174200 Os03g0174200|AK103762
         (398 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G52110.1  | chr3:19323925-19325632 REVERSE LENGTH=363          130   1e-30

>AT3G52110.1 | chr3:19323925-19325632 REVERSE LENGTH=363
          Length = 362

 Score =  130 bits (327), Expect = 1e-30,   Method: Compositional matrix adjust.
 Identities = 129/433 (29%), Positives = 179/433 (41%), Gaps = 114/433 (26%)

Query: 4   MEPKDIDWSRVVSRYVRDETYEGIEAPHWADLTDPEAGVAAVDDEAWFCRPDCRHPKTVE 63
           MEP  IDW R+ SR+V D  YE I AP W D   P     ++DD+AWFC+P+C HPK  E
Sbjct: 1   MEPAHIDWKRIDSRFVEDVFYEHIRAPKWFDFLAP-THFDSIDDDAWFCKPECNHPKRPE 59

Query: 64  DFLKMSPSPKGKLLRSVSAMMMPFGERDTNLRDGNNNLKRRGAVAGSGIAATFTXXXXXX 123
           DF     S K   L            RDTN      N +RRG                  
Sbjct: 60  DFFSTPTSSKHPSL------------RDTNETLTEQNQRRRGYALSPSTPNN-------- 99

Query: 124 XXXXRFQDDSENQDXXXXXXXXXXXXXXXXXXXXXRWAKNAKDAIKSSAEKRPGNAEKEA 183
                   +SENQ+                       AK+ + AIKS++ K+  N E   
Sbjct: 100 -------QESENQNPNLATPPSYQ-------------AKSWRAAIKSTSVKK-MNKE--- 135

Query: 184 LLSKNAAPRQLKSTLSARNLFSGKDILGQISDFYNELKRMA--------GGNGSRPGSEA 235
                  PR LKST SARNLFSG+DI G ISDF  ELKR+A         G      S  
Sbjct: 136 ------VPR-LKSTQSARNLFSGRDIFGHISDFCYELKRLATRVTEREDTGKSEVKESHQ 188

Query: 236 MEELSSNPINEGDVAEKKVDCGC--GTGDQVPSEEAIK---------EKSRQETAEKSPS 284
           + E+ + P +  D+  KK        + D++P    +K          K R + AE  P 
Sbjct: 189 VGEIVNQPYSVHDLELKKERKPLLEVSKDKLPESTDVKGSTFKENRRRKKRVDDAENIPV 248

Query: 285 TM-------------KGKKMG--------LKVEAAKPT-RSSVLKEVKATPPTPQRFPSP 322
           ++             + K+M         LK+E  K       L++++  PP+PQ F   
Sbjct: 249 SLNVETVKNKGEEGRRKKRMDDAENIPVPLKLETIKNKGHERFLQQIRTNPPSPQCF--- 305

Query: 323 STNRVKNVKAGGMSMASSPLKKPLKEKGTPSKDLENSKDAKRQPFGVKDMNNTKACDAEG 382
           S NR   +             KPL+ K  P++ L+  +D   +    K+  + ++ +A  
Sbjct: 306 SENRTATL-------------KPLRTK--PTEVLKRKEDEAEEE---KNRKSGESKEATR 347

Query: 383 SSSMFWFLKPCTF 395
              + WFLKPCT 
Sbjct: 348 GLDVLWFLKPCTL 360



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.310    0.127    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,612,895
Number of extensions: 378544
Number of successful extensions: 1224
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1223
Number of HSP's successfully gapped: 2
Length of query: 398
Length of database: 11,106,569
Length adjustment: 101
Effective length of query: 297
Effective length of database: 8,337,553
Effective search space: 2476253241
Effective search space used: 2476253241
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 113 (48.1 bits)