BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0158300 Os03g0158300|AK066591
         (359 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G64770.3  | chr1:24057549-24059405 FORWARD LENGTH=368          231   4e-61
AT1G55370.2  | chr1:20674852-20676071 FORWARD LENGTH=355           77   1e-14

>AT1G64770.3 | chr1:24057549-24059405 FORWARD LENGTH=368
          Length = 367

 Score =  231 bits (589), Expect = 4e-61,   Method: Compositional matrix adjust.
 Identities = 117/261 (44%), Positives = 164/261 (62%), Gaps = 10/261 (3%)

Query: 35  LEESFGRKGLRFAADPATGAPTAELSVRNGSSLQLRLADGLVTSYRPKVYWKDDGCREVL 94
           LE+ FGRKG++F+   +   P  EL VRNGSSL+L L+D  V SY+PKVYWKD+G  EVL
Sbjct: 33  LEDKFGRKGIKFSE--SNNIPMVELKVRNGSSLKLSLSDAHVLSYKPKVYWKDEGFEEVL 90

Query: 95  HTXXXXXXXXXXXXXXXLALSEVSSSGAAESLLVGSEWXXXXXXXXXXXXXXXELGCTKG 154
           +T                 +          S++ G +W               EL CT  
Sbjct: 91  YTVDGDESRGGVGV----VIVNGEEPKGGSSVISGCDWSVKDTDSDAIDALQIELSCT-- 144

Query: 155 SGTLEVTYVVTLYPLSMATAVMVKNNGKKPVSLTSAMLSHIKFDKRRGTAVEGLRGCPYC 214
           +G L++TY+V+LYP+SMATA++VKNNG+KPV+L   ++S+++F KR G  ++GL+GC YC
Sbjct: 145 AGVLDITYIVSLYPVSMATALVVKNNGRKPVTLKPGIMSYLRFKKRSGAGIQGLKGCSYC 204

Query: 215 SHPPPAAGFALLTPAEAMKREDXXXXXXXXXEEPRQGVWTVEDNLYTILKKKVSRVYAAP 274
            +PP ++ F LL+P+EAMK E          E+P  G+W VED++ T+L+KK+SR+Y AP
Sbjct: 205 PNPPLSSPFELLSPSEAMKAESSGWFGSEEGEKP--GIWAVEDSVITLLEKKMSRIYGAP 262

Query: 275 PEERKKRIYSTAPSKFTTIDQ 295
           P ER K +Y+T PSKF TIDQ
Sbjct: 263 PAERLKAVYNTPPSKFETIDQ 283



>AT1G55370.2 | chr1:20674852-20676071 FORWARD LENGTH=355
          Length = 354

 Score = 77.0 bits (188), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 71/304 (23%), Positives = 125/304 (41%), Gaps = 37/304 (12%)

Query: 57  AELSVRNGSSLQLRLADGLVTSYRPKVYWKDDGCREVLHTXXXXXXXXXXXXXXXLALSE 116
           A L + NGSS  + L  G++TSY+ +V+    G  E+LHT                 +S 
Sbjct: 85  ARLKLDNGSSANVMLTRGMITSYKVRVW--HGGKVELLHTWVEQEEEEVVIRG---GVSS 139

Query: 117 VSSSGAAESLLVGSEWXXXXXXXXXXXXXXXELG-CTKGSGTLEVTYVVTLYPLSMATAV 175
              S  ++ +   S+W               EL    K    +E+  +++L   +++  +
Sbjct: 140 AFRSSDSDEI---SDWRLQGISGDSKDCVQMELRRSDKKIKEIELKQIISLRENTLSIEL 196

Query: 176 MVKNNGKKPVSLTS-AMLSHIKFDKRRGTAVEGLRGCPYCSHPPPAAGFALLTPAEAMKR 234
            + N G  P+ L   +++S++       T   GL G  +    P    F ++   +    
Sbjct: 197 SMTNKGISPIKLEGCSLVSYLTVSTPEATYAVGLEGSDFVETTPFLPRFGVVQGEK---- 252

Query: 235 EDXXXXXXXXXEEPRQGVWTVEDNLYTILKKKVSRVYAAPPEERKKRIYSTAPSKFTTID 294
                      EE + G    E++ Y  L +++SR            IY+ AP  FT ID
Sbjct: 253 -----------EEEKPGFGGEEESNYKQLNREMSR------------IYTCAPKSFTVID 289

Query: 295 QNSGLGFRVVRMGYEDMYLCSPGEMYRKFGKDYFLCTGTASMLVPVVVNPGEEWRAAQVI 354
           +       V R G+E++Y+ SPG     + K  ++C G +S+L P+ +  G  WR    +
Sbjct: 290 RGRRNSVVVGREGFEEVYMYSPGSRLESYTKSAYVCIGPSSLLSPISLESGCVWRGVLHL 349

Query: 355 EHDN 358
            + N
Sbjct: 350 HNPN 353



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.316    0.133    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,526,110
Number of extensions: 232878
Number of successful extensions: 416
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 414
Number of HSP's successfully gapped: 2
Length of query: 359
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 259
Effective length of database: 8,364,969
Effective search space: 2166526971
Effective search space used: 2166526971
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 112 (47.8 bits)