BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0144000 Os03g0144000|AK065797
         (504 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G04650.1  | chr1:1294893-1298903 REVERSE LENGTH=998            249   3e-66

>AT1G04650.1 | chr1:1294893-1298903 REVERSE LENGTH=998
          Length = 997

 Score =  249 bits (636), Expect = 3e-66,   Method: Compositional matrix adjust.
 Identities = 154/487 (31%), Positives = 258/487 (52%), Gaps = 17/487 (3%)

Query: 20  NIAQITACTIQSLCKIGTHAASSGGSQVILLNISWKGIISLLQLGKGMVEEKVNVREIIL 79
           N+  +    ++SL ++G  AA  GG+ V +LN SWKG+I+LLQL K  +  KV+V EIIL
Sbjct: 229 NLVSMITLGVKSLSELGMLAARDGGNLVAILNTSWKGVITLLQLDKQTLVSKVDVGEIIL 288

Query: 80  IPISVAIESLRVATERWCVPLQEVLGTAEARRAFLPIKYFLTNAVRICSIYPSETMAIYK 139
             IS+  +SLR A E W   ++E +   EARR FLP+K++L NAV++ +++PS+   + K
Sbjct: 289 KLISLIKDSLRFAAEAWSCSVKENISATEARRVFLPVKFYLINAVKVVALFPSQASMVSK 348

Query: 140 SIIRCALLISHSSILFCRNPQLKAAXXXXXXXXXXXXXXXXDTLMKSTEVSPESKCQLVQ 199
            I  C L+IS   +   +    K+A                  L+ + E++ E +  L+ 
Sbjct: 349 DIALCILMISAFKVSLSQQTHGKSASEVMTDLLEKTTVDLLGALLNAAELTQEFRLTLLD 408

Query: 200 -YFLEKVRTGNPEYTGEVDHTMNFATLGCIFSVDSDVDNINRALLPAEFTVFLHFLNASP 258
             F+++      +      HT    +L  I S+  +     R LL A   +F   +  S 
Sbjct: 409 SLFVDEFSNQICKKQSHDSHTK--TSLVDILSLSVESATSARDLLLARVVLFQSVMRYSF 466

Query: 259 WLREEVVIELCKKLHLFLNILTLEDVYSYVLGCQIPALSSDDDSPKVVWQPVYTSLIQAL 318
            L ++  + +  KL   L+IL  ++VYS VL  Q+P   +D     V+W+ +Y++L+ +L
Sbjct: 467 ELDKDAKLAITTKLQWLLDILADKEVYSSVLSSQLPM--ADGSGKIVIWESMYSALLLSL 524

Query: 319 KTFMIXXXXXXXXWSEFEVFLLENLFHPHFLCLEILTELWCFFMHYAEAETSTYLINQLF 378
           KT MI        W E E FLL+NL HPHFLC +I+ ELWCF++ +A  +    +INQL 
Sbjct: 525 KTLMI-ILSSTPAWEELETFLLQNLLHPHFLCWQIVMELWCFWVRHATDDLVVDMINQLC 583

Query: 379 LLLKTLASPEEVLAPLSALRKLARASCNILSYASSATIDQIYTMLN--DSSSKSSILYLA 436
             + ++ S E  L P S LR+  ++ C +L+++  +   Q+Y  ++    S  +  +YLA
Sbjct: 584 TFIMSMPSSETPLCPDSVLRRTTKSICFLLTHSPKSLTVQVYKHISTESRSDHAPDVYLA 643

Query: 437 LLMEGFPFDSLSRGVKEHAVKTLFTSFAGYLQNQNYFKNHGEINLPTSSSS-GIIGFPVH 495
           LL++GFP + L   +K  A + +F  F  +++  +          P++SS   ++G PV 
Sbjct: 644 LLLDGFPLNFLPDRIKNDAKRQIFADFFNFIEKFD--------EKPSNSSRYTLLGAPVF 695

Query: 496 ALASAFQ 502
            +++  +
Sbjct: 696 TVSACLR 702



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.135    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 9,518,652
Number of extensions: 362439
Number of successful extensions: 756
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 752
Number of HSP's successfully gapped: 1
Length of query: 504
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 401
Effective length of database: 8,282,721
Effective search space: 3321371121
Effective search space used: 3321371121
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 114 (48.5 bits)