BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0143000 Os03g0143000|AK073102
         (195 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G26200.1  | chr2:11152875-11156330 FORWARD LENGTH=566          243   5e-65
AT1G54650.2  | chr1:20405813-20407686 REVERSE LENGTH=302           96   1e-20

>AT2G26200.1 | chr2:11152875-11156330 FORWARD LENGTH=566
          Length = 565

 Score =  243 bits (620), Expect = 5e-65,   Method: Compositional matrix adjust.
 Identities = 116/162 (71%), Positives = 132/162 (81%), Gaps = 2/162 (1%)

Query: 21  QFFKDRHYLDKEWGKYFEGQGGGKMVILEVGCGAGNTIFPLISTYPDIFVHACDFSPRAV 80
           +FFKDRHYLDKEW  YF     GK VILEVGCGAGNTIFPLI+TYPDIFV+ACDFSPRAV
Sbjct: 54  RFFKDRHYLDKEWNSYF--SVSGKSVILEVGCGAGNTIFPLIATYPDIFVYACDFSPRAV 111

Query: 81  DLVKKHKDYRPDRVNAFACDITSEQLTENVQPSSVDVVTMIFMLSAVAPAKMPLVLQNVR 140
           +LVK H +Y   RV AFACD+T + L +++ PSSVD+VTMIF+LSAV+P KM  VLQN+R
Sbjct: 112 ELVKAHDEYTETRVCAFACDLTGDGLDKHISPSSVDIVTMIFVLSAVSPEKMSSVLQNIR 171

Query: 141 TVLKNGGRVLFRDYAFGDLAQERLMSKGQQISENFYVRGDGT 182
            VLK  G +LFRDYA GDLAQER   K Q+ISENFYVRGDGT
Sbjct: 172 KVLKPNGCILFRDYAVGDLAQERFSGKDQRISENFYVRGDGT 213



>AT1G54650.2 | chr1:20405813-20407686 REVERSE LENGTH=302
          Length = 301

 Score = 95.5 bits (236), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 63/191 (32%), Positives = 95/191 (49%), Gaps = 29/191 (15%)

Query: 20  GQFFKDRHYLDKEWGKYFEGQGGGKMVILEVGCGAGNTIFPLISTYPDIFVHACDFSPRA 79
           G+FFK+R YL KE+ +      G    +LE+GCG G+T+ P++    +I V+ACD S  A
Sbjct: 61  GKFFKERRYLLKEFPELV--SCGENSKLLEIGCGNGSTVLPILRGSKNITVYACDCSSDA 118

Query: 80  VDLVKKHKDY---RPDRVNAFACDITSEQLTENVQPS----------------------- 113
           +   K++ D      D  ++F CD ++ +  + V                          
Sbjct: 119 LVRTKENIDRAISSVDNFHSFCCDFSTSEFPDWVACDRCRDKFMLNHSGFGGSESKHCIG 178

Query: 114 SVDVVTMIFMLSAVAPAKMPLVLQNVRTVLKNGGRVLFRDYAFGDLAQERLMSKGQQISE 173
            VD VT+IF LSAV   +MP  ++    VLK GG +LFRDY   D+   R   + +++  
Sbjct: 179 GVDFVTLIFTLSAVPKERMPRAIKECFAVLKPGGLLLFRDYGLYDMTMLRFEPE-KRVGF 237

Query: 174 NFYVRGDGTVS 184
             YVR DGT+S
Sbjct: 238 REYVRSDGTLS 248



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.326    0.140    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,362,941
Number of extensions: 183162
Number of successful extensions: 509
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 506
Number of HSP's successfully gapped: 2
Length of query: 195
Length of database: 11,106,569
Length adjustment: 93
Effective length of query: 102
Effective length of database: 8,556,881
Effective search space: 872801862
Effective search space used: 872801862
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 109 (46.6 bits)