BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0119000 Os03g0119000|AK071417
(256 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G51880.1 | chr5:21089395-21090775 FORWARD LENGTH=251 268 2e-72
>AT5G51880.1 | chr5:21089395-21090775 FORWARD LENGTH=251
Length = 250
Score = 268 bits (684), Expect = 2e-72, Method: Compositional matrix adjust.
Identities = 135/219 (61%), Positives = 157/219 (71%), Gaps = 11/219 (5%)
Query: 49 RWPPVKPKKDLQVNRLKGTHLLTVPNFFTSAEAKAFVDIAENIGFTHQGSLGPLKGEAYR 108
+WP +K K +L V+ LK L TV N TS E+KAFV IAE++GFTHQGS GP GEAYR
Sbjct: 32 KWPKIKLKSNLNVSYLKNHDLFTVENCLTSDESKAFVKIAESLGFTHQGSRGPAYGEAYR 91
Query: 109 DNDRISVTDPLLAQTIWESGINKIFEDISISGKVATSLNPNIRFYRYTEGQRFGRHIDES 168
DN RISV DP+LA T+W+SG++ +F DI I KVA LNPNIRFYRY+ GQ FGRHIDES
Sbjct: 92 DNHRISVNDPVLADTLWQSGLSNLFTDIKIRRKVAVGLNPNIRFYRYSAGQHFGRHIDES 151
Query: 169 VDLGDGSRTLYTLLIYL-----------SGKGSAKESSGQALVGGETVFYDHRGGVVAEV 217
DL DG+RT YTLLIYL S + SS + LVGGETVFY R +VAEV
Sbjct: 152 ADLEDGNRTYYTLLIYLSGNSTKSKSKSSSSKTNDSSSAEPLVGGETVFYGSRNSIVAEV 211
Query: 218 APVQGLALLHLHGAKCMLHEARVVKKNIKYVLRSDVVFA 256
APV+G+AL H+HG KCMLHE R V K +KYV RSDVVFA
Sbjct: 212 APVEGMALFHIHGDKCMLHEGRNVSKGVKYVFRSDVVFA 250
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.319 0.137 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,932,767
Number of extensions: 197546
Number of successful extensions: 410
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 409
Number of HSP's successfully gapped: 1
Length of query: 256
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 160
Effective length of database: 8,474,633
Effective search space: 1355941280
Effective search space used: 1355941280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 110 (47.0 bits)