BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0116500 Os03g0116500|AK101870
         (441 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G61140.1  | chr3:22626335-22628895 FORWARD LENGTH=442          589   e-169
AT4G24820.1  | chr4:12790471-12792599 REVERSE LENGTH=388           75   9e-14
AT3G12850.1  | chr3:4088374-4090303 REVERSE LENGTH=368             67   2e-11

>AT3G61140.1 | chr3:22626335-22628895 FORWARD LENGTH=442
          Length = 441

 Score =  589 bits (1519), Expect = e-169,   Method: Compositional matrix adjust.
 Identities = 280/412 (67%), Positives = 336/412 (81%), Gaps = 1/412 (0%)

Query: 29  VSAEQLDVEAYAAQYTGRTRLARLLFIAERCGVE-AVELEALRMAYDEIKRGEDTMFHRE 87
           +S E LD+EAYAA Y GRT++ RLLFIA  CG   A++ +ALRMAYDEIK+GE+T   RE
Sbjct: 29  ISGEPLDIEAYAALYKGRTKIMRLLFIANHCGGNHALQFDALRMAYDEIKKGENTQLFRE 88

Query: 88  VTNKINGRLGPKYALDQAWTDSVNRRAEQRKEKLESELNGYRTNLIKESIRMGYNDIGDF 147
           V NKI  RLG KY +D AW ++V+RRAEQ+K KLE+EL+ YRTNLIKESIRMGYND GDF
Sbjct: 89  VVNKIGNRLGEKYGMDLAWCEAVDRRAEQKKVKLENELSSYRTNLIKESIRMGYNDFGDF 148

Query: 148 FYAHGHLSDAFKSYIRTRDYCTTSKHIVQMCMNVILVSIELGQFPHVSNYVSKAEQTPDT 207
           +YA G L DAFK+YIRTRDYCTT+KHI+ MCMN ILVSIE+GQF HV++YV+KAEQ P+T
Sbjct: 149 YYACGMLGDAFKNYIRTRDYCTTTKHIIHMCMNAILVSIEMGQFTHVTSYVNKAEQNPET 208

Query: 208 LDPIIVXXXXXXXXXXXXXXXXXXXXXXXFVETGHELGNNYSEVIAPQDVAVYGALCALA 267
           L+P++                        F++   ELGN+Y+EVIAPQD+A YG LCALA
Sbjct: 209 LEPMVNAKLRCASGLAHLELKKYKLAARKFLDVNPELGNSYNEVIAPQDIATYGGLCALA 268

Query: 268 SFDRSDLKSKVIDNSNFRNFLELVPEVRELVNDFYSSRYGSCLEHLEKLKTNLLLDIHLH 327
           SFDRS+LK KVIDN NFRNFLELVP+VREL+NDFYSSRY SCLE+L  LK+NLLLDIHLH
Sbjct: 269 SFDRSELKQKVIDNINFRNFLELVPDVRELINDFYSSRYASCLEYLASLKSNLLLDIHLH 328

Query: 328 DHVETLYMDIRHKAIIQYTLPFISVDLNTMAAAFMTSVSMLEKELAALITENKIQARIDS 387
           DHV+TLY  IR KA+IQYTLPF+SVDL+ MA AF TSVS LEKEL ALIT+N+IQARIDS
Sbjct: 329 DHVDTLYDQIRKKALIQYTLPFVSVDLSRMADAFKTSVSGLEKELEALITDNQIQARIDS 388

Query: 388 HNKILYARHADQRNATFQRVLQTGNEFERDVKSLLLRANLIKHDFNQRAGQR 439
           HNKILYARHADQRNATFQ+VLQ GNEF+RDV+++LLRANL+KH+++ R+ ++
Sbjct: 389 HNKILYARHADQRNATFQKVLQMGNEFDRDVRAMLLRANLLKHEYHARSARK 440



>AT4G24820.1 | chr4:12790471-12792599 REVERSE LENGTH=388
          Length = 387

 Score = 74.7 bits (182), Expect = 9e-14,   Method: Compositional matrix adjust.
 Identities = 41/163 (25%), Positives = 73/163 (44%)

Query: 250 EVIAPQDVAVYGALCALASFDRSDLKSKVIDNSNFRNFLELVPEVRELVNDFYSSRYGSC 309
           E+   +    Y  L ++ + DR  LK KV+D       L  +P + E +N  Y  +Y + 
Sbjct: 210 EIFPYETFIFYTVLTSIITLDRVSLKQKVVDAPEILTVLGKIPFLSEFLNSLYECQYKAF 269

Query: 310 LEHLEKLKTNLLLDIHLHDHVETLYMDIRHKAIIQYTLPFISVDLNTMAAAFMTSVSMLE 369
                 +   +  D +L+ H      ++R     Q+   + SV +  MA AF  SV  ++
Sbjct: 270 FSAFAGMAVQIKYDRYLYPHFRFYMREVRTVVYSQFLESYKSVTVEAMAKAFGVSVDFID 329

Query: 370 KELAALITENKIQARIDSHNKILYARHADQRNATFQRVLQTGN 412
           +EL+  I   K+  +ID    +L     D +NA +Q  ++ G+
Sbjct: 330 QELSRFIAAGKLHCKIDKVAGVLETNRPDAKNALYQATIKQGD 372



>AT3G12850.1 | chr3:4088374-4090303 REVERSE LENGTH=368
          Length = 367

 Score = 67.0 bits (162), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 55/174 (31%), Positives = 80/174 (45%), Gaps = 24/174 (13%)

Query: 34  LDVEAYAAQYTGRTRLARLLFIAERCG----VEAVELEALRMAYDEIKRGE---DTMFHR 86
           LDVEAY+  Y GR  + RLL IAE        + +ELEALR+AYD I+      D     
Sbjct: 11  LDVEAYSRNYEGRNLVLRLLHIAENSVGPMFTDDLELEALRLAYDLIRNDGIVCDMRLFT 70

Query: 87  EVTNKINGRLGPKYALDQAWTDSVNRRAEQRKEKLESELNGYRTNLIKE--SIRMGYNDI 144
            +  KI GRLG  Y LD+ W  + N    ++   L ++L      L K+  ++R  +   
Sbjct: 71  VIAEKIQGRLGEGYLLDEKWRLTKNNNFVKQDSILRNDLESATDELEKDRLALRQYW--- 127

Query: 145 GDFFYAHGHLSDAFKSYIRTRDYCTTSKHIVQMCMNVILVSIE---LGQFPHVS 195
               Y  G ++  F+ Y R           +   M  +LV++E    G+ PH S
Sbjct: 128 --LHYHCGQMAIVFRPYPR-------QVQTIDAYMATLLVALETRNFGELPHRS 172



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.320    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,895,985
Number of extensions: 298261
Number of successful extensions: 878
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 874
Number of HSP's successfully gapped: 3
Length of query: 441
Length of database: 11,106,569
Length adjustment: 102
Effective length of query: 339
Effective length of database: 8,310,137
Effective search space: 2817136443
Effective search space used: 2817136443
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 113 (48.1 bits)