BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0102400 Os03g0102400|AK121721
         (493 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G61450.1  | chr5:24710240-24712882 REVERSE LENGTH=448          513   e-145

>AT5G61450.1 | chr5:24710240-24712882 REVERSE LENGTH=448
          Length = 447

 Score =  513 bits (1321), Expect = e-145,   Method: Compositional matrix adjust.
 Identities = 257/419 (61%), Positives = 308/419 (73%), Gaps = 25/419 (5%)

Query: 79  RNASSKYDFVKVKVWLGENADHYYVLSRFLLCRMLTVTKIPNHVXXXXXXXXXXXXVDNS 138
           RNASSKYDFVKVKVWLG+NADHYYVLSRFL+CRMLTVTKIPNH             +DNS
Sbjct: 46  RNASSKYDFVKVKVWLGDNADHYYVLSRFLVCRMLTVTKIPNHEAIKISLELKKLLIDNS 105

Query: 139 LLDVSQSDLEANLFKLMEKRGYGEDYINRYKMMTRFHHQRVPLVVLVCGTACTGKSTIAT 198
           LLDVSQSDLE NLFKLME+RGYGE+YINRY MMT+FHHQRVPLV+LVCGTAC GKSTIAT
Sbjct: 106 LLDVSQSDLETNLFKLMERRGYGEEYINRYNMMTKFHHQRVPLVILVCGTACVGKSTIAT 165

Query: 199 QLAGRLNLPNVLQTDMVYELLRTSTDAPLTSVPVWARDFDSPEELITEFCRECRVVRKGL 258
           QLA RLNLPNVLQTDMVYELLRT+TDAPLTS PVW R+F S EELITEFCRECR+VRKGL
Sbjct: 166 QLAQRLNLPNVLQTDMVYELLRTATDAPLTSTPVWTREFGSSEELITEFCRECRIVRKGL 225

Query: 259 AGDLKKAMKDGKPIIIEGIHLDPSIYLMDEEKRDDNSKMEKKVAESENS--------PAT 310
            GDLKKAMKDGKPIIIEG HLDPSIYLM++E +  ++  EK  +E+ +S        P  
Sbjct: 226 GGDLKKAMKDGKPIIIEGRHLDPSIYLMNDENKTPSNDPEKSSSETNSSKDLTSDKNPEA 285

Query: 311 VKSKTEKQQENELHEKRMDDSQECMSEEGRISEGLSCAKSHVISSSDPACSKEKNPRAEG 370
             S T K+ +N   +    +      E   +SE ++       SS D   +++K+  A+G
Sbjct: 286 SSSNT-KETDNSAVKPHSHEEAVASVEANNLSEKVTQCTIDAESSKD---AEKKSKSADG 341

Query: 371 EGHKDLDLQKNNATKKDKPAAKPIIVPIVLRMSDFDHKALLEEWIATRASRDNCLPQDHR 430
            G             K K   +PI++ IVL+MS+FDHKALLEEWI++R S +    ++  
Sbjct: 342 SG-------------KTKSGPEPIVISIVLKMSEFDHKALLEEWISSRTSGEKYTTKEKD 388

Query: 431 KLINNLKLIQDYLCSFESQGLTVVDISANSFPQTLDWLHSYLLQCIERGLLAACSESPK 489
           +LI NLK I+DYLCSF SQG+TVV+ISA +FPQTLDWLH+YLLQ IE G+ ++ +E+PK
Sbjct: 389 RLITNLKTIEDYLCSFNSQGVTVVNISATTFPQTLDWLHNYLLQRIEEGIRSSENEAPK 447



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.316    0.132    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 9,921,679
Number of extensions: 400586
Number of successful extensions: 2255
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 2258
Number of HSP's successfully gapped: 1
Length of query: 493
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 390
Effective length of database: 8,282,721
Effective search space: 3230261190
Effective search space used: 3230261190
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 114 (48.5 bits)