BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os02g0822300 Os02g0822300|J080043B20
(156 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G04430.2 | chr5:1250602-1253523 REVERSE LENGTH=335 170 2e-43
>AT5G04430.2 | chr5:1250602-1253523 REVERSE LENGTH=335
Length = 334
Score = 170 bits (431), Expect = 2e-43, Method: Compositional matrix adjust.
Identities = 86/158 (54%), Positives = 112/158 (70%), Gaps = 12/158 (7%)
Query: 1 IYLILSKLSEDVHYPPNLSSPFPYAGLGFPSYPGVPVGYMIPQVPYN--NAVNYGPNGYG 58
I LIL+KL+ED HY N+ SP+ YAGL + + G P Y +P V N+VNY PNG G
Sbjct: 184 IDLILAKLTEDDHYSQNVHSPYSYAGLFYSGFHGPPYAYALPSVATAGYNSVNYAPNGSG 243
Query: 59 GRYQNNKPSTPMRSPANNDAQDSLTIGIADEHIGAVVGRAGRNITEIIQASGARIKISDR 118
G+YQN+K +A ++TIG+ADEHIG V+GR GRNI EI Q +GARIKISDR
Sbjct: 244 GKYQNHK----------EEASTTVTIGVADEHIGLVLGRGGRNIMEITQMTGARIKISDR 293
Query: 119 GDFIAGTSERKVTITGTSEAIQAAESMIMQRVTASSER 156
GDF++GT++RKV+ITG AIQ AE+MI Q+V +++ER
Sbjct: 294 GDFMSGTTDRKVSITGPQRAIQQAETMIKQKVDSATER 331
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.313 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,488,544
Number of extensions: 159460
Number of successful extensions: 364
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 372
Number of HSP's successfully gapped: 3
Length of query: 156
Length of database: 11,106,569
Length adjustment: 90
Effective length of query: 66
Effective length of database: 8,639,129
Effective search space: 570182514
Effective search space used: 570182514
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 107 (45.8 bits)