BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os02g0300700 Os02g0300700|AK069347
         (142 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G04520.1  | chr2:1574802-1575239 REVERSE LENGTH=146            207   1e-54
AT5G35680.3  | chr5:13858085-13858589 REVERSE LENGTH=151          191   1e-49

>AT2G04520.1 | chr2:1574802-1575239 REVERSE LENGTH=146
          Length = 145

 Score =  207 bits (528), Expect = 1e-54,   Method: Compositional matrix adjust.
 Identities = 105/145 (72%), Positives = 114/145 (78%), Gaps = 3/145 (2%)

Query: 1   MPXXXXXXXXXXXXXXXEADDEKRELVFKEDGQEYAQVARMLGNGRCEAQCIDGTKRLCH 60
           MP               EADDEKREL+FKEDGQEYAQV RMLGNGRCEA CIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMNDEARLLKAYGEIPDHVRLNEGVV 120
           IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYM+DEARLLKAYGE+P++ RLNEG+V
Sbjct: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGIV 120

Query: 121 ---XXXXXXXXXXYIQFEDEDIDKI 142
                        Y++FEDEDID+I
Sbjct: 121 GDLEDDDDNNDDDYVEFEDEDIDRI 145



>AT5G35680.3 | chr5:13858085-13858589 REVERSE LENGTH=151
          Length = 150

 Score =  191 bits (484), Expect = 1e-49,   Method: Compositional matrix adjust.
 Identities = 91/119 (76%), Positives = 99/119 (83%)

Query: 1   MPXXXXXXXXXXXXXXXEADDEKRELVFKEDGQEYAQVARMLGNGRCEAQCIDGTKRLCH 60
           MP               EADDEKREL+FKEDGQEYAQV RMLGNGRC+  CIDG KRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMNDEARLLKAYGEIPDHVRLNEGV 119
           IRGKMHKKVWIAAGDI+LVGLRDYQDDKADVILKYM+DEARLLKAYGE+P++ RLNEG+
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGI 119



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.141    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,739,201
Number of extensions: 103610
Number of successful extensions: 168
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 167
Number of HSP's successfully gapped: 2
Length of query: 142
Length of database: 11,106,569
Length adjustment: 89
Effective length of query: 53
Effective length of database: 8,666,545
Effective search space: 459326885
Effective search space used: 459326885
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 106 (45.4 bits)